综合医院分枝杆菌气溶胶采样及巢式PCR检测方法研究

Study on aerosol sampling and nested PCR detection of Mycobacterium tuberculosis in general hospital

目的:调查综合医院空气中分枝杆菌的污染状况。方法:于2010年7月选取广东省4家综合医院,采用Bio-Sampler生物冲击式采样器采集内科和儿科侯诊室的空气和集中空调系统送风气溶胶,巢式PCR扩增结核分枝杆菌复合群IS6110和分枝杆菌Hsp65基因,琼脂糖凝胶电泳检测扩增产物。结果:空气中结核分枝杆菌复合群的阳性率为10.2%(5/49);分枝杆菌的阳性率为26.5%(13/49)。内科侯诊室空气中结核分枝杆菌复合群和分枝杆菌的阳性率高于儿科侯诊室但差异无统计学意义。集中空调系统送风中结核分枝杆菌复合群的阳性率为11.4%(4/35)。结论:液体冲击式气溶胶采样器可用于采集分枝杆菌气溶胶,我国综合医院侯诊室空气和集中空调系统存在分枝杆菌包括结核分枝杆菌污染。

Objective： To investigate the contamination condition of Mycobacterium tuberculosis in general hospital.Methods： The aerosol samples of mycobacteria were collected by BioSampler in waiting room of medical and pediatrics departments in 4 general hospitals in Guangdong.Nested-PCR based on IS6110 and Hsp65 gene was used to detect Mycobacterium tuberculosis complex and mycobacteria respectively.Results： The positive rate of Mycobacterium tuberculosis complex and mycobacteria were 10.2%（5/49） and 26.5%（13/49） respectively.There was no significant difference in the positive rate of Mycobacterium tuberculosis complex and mycobacteria between waiting room of medical and pediatrics departments in general hospital,though the tested positive rates in medical department were higher than those in pediatrics department.The positive rate of Mycobacterium tuberculosis complex in supply air of central air conditioning system was 11.4%（4/35）.Conclusion： The impinger is an available method to take sample of the mycobacterium aerosol.There is mycobacteria including Mycobacterium tuberculosis complex contamination in central air conditioning system and waiting rooms of general hospital.