海芦笋膳食纤维提取技术

Extraction Techniques of Dietary Fiber from Sea Asparagus

[目的]研究从海芦笋提取膳食纤维的工艺条件,并制备可溶性和不溶性膳食纤维。[方法]采用酶与化学法相结合的方法,制备纤维,不溶性膳食纤维先用0.75%NaOH溶液碱煮30 min,再用1.5%HCl溶液酸煮20 min,将可溶性和不溶性膳食纤维混合。[结论]优化工艺下制备海芦笋膳食纤维,得率为21.28%,其中可溶性膳食纤维∶不可溶性膳食纤维为1∶3。

[Objective] The objective was to study the technology condition for extracting the dietary fiber from the sea asparagus and prepare the soluble and insoluble dietary fiber.[Method] The method of combining the enzyme and chemical was used to prepare the 2 kinds of dietary fiber.[Result] Under the conditions as pH in cellulose of 4-5,pH in neutral protease solution of 6-7,temperature of 48-50 ℃,cellulose of 800 U/g,protease of 2 000 U/g,enzyme solution time of 2 h,the best enzymatic hydrolysis results were obtained.The isolated soluble dietary fiber and insoluble dietary fiber were obtained after extracting the crude fiber with 2.25% sodium carbonate solution(40 times) for 1h at 50 ℃.The insoluble dietary fiber was first boiled with 0.75% sodium hydroxide solution for 30 min,then with 1.5% hydrochloric acid solution for 20 min.The insoluble dietary fiber could be mixed with the soluble dietary fiber.[Conclusion] The preparation of the dietary fiber from the sea asparagus under the optimized process could get the yield of 21.28%,in which the ratio of soluble dietary and fiber insoluble dietary fiber was 1∶ 3.