摘要
目的探讨DNA甲基转移酶1、3A和3B在宫颈癌发生发展中的作用。方法应用real-time PCR技术检测10例宫颈癌、10例宫颈上皮内瘤变和10例正常宫颈组织DNMT1、3A和3BmRNA的表达。结果宫颈癌组织、宫颈上皮内瘤变和正常宫颈中DNMT 1mRNA对GAPDH mRNA的相对丰度值分别是0.30±0.1、0.26±0.12和0.10±0.04,宫颈癌组织组织中与正常宫颈中DNMT 1表达有显著差异(P<0.05);宫颈癌组织、宫颈上皮内瘤变和正常宫颈中DNMT3A mRNA对GAPDH mRNA的相对丰度值分别是0.94±0.27、0.51±0.24和0.13±0.04,三组相比较有显著性差异(P<0.05);宫颈癌组织、宫颈上皮内瘤变和正常宫颈中DNMT 3B mRNA对GAPDH mR-NA的相对丰度值分别是0.30±0.09、0.28±0.11和0.11±0.04,宫颈癌组织组织中与正常宫颈中DNMT 3B表达有显著差异(P<0.05)。结论 DNMT1、3A和3B参与了宫颈癌的发生,并在宫颈上皮内瘤变时就已经起了一定作用。
Objective: To study the relationship between DNMT1,3A and 3BmRNA expression and oncogenesis of uterine cervical carcinoma.Methods: The expressions of DNMT1,3A and 3B mRNA were detected by real-time PCR in 10 cases of cervical carcinoma,10 cases of cervical intraepithelial neoplasia(CIN) and 10 cases of normal cervical tissue.Results: The relative abundance of DNMT 1mRNA to GAPDH mRNA in cervical carcinoma,in CIN and in normal cervica were 0.30±0.1,0.26±0.12 and 0.10±0.04 respectively.The DNMT 1 mRNA was found to be increased in cervical carcinoma(P0.05,vs normal cervical tissue).The relative abundance of DNMT 3A?mRNA to GAPDH mRNA in cervical carcinoma,in CIN and in normal cervica were 0.94±0.27,0.51±0.24 and 0.13±0.04,respectively.There were significant differences among three groups(P0.05).The relative abundance of DNMT 3B mRNA to GAPDH mRNA in cervical carcinoma,in CIN and in normal cervica were 0.30±0.09,0.28±0.11 and 0.11±0.04 respectively.The DNMT 3B mRNA was found to be increased in cervical carcinoma(P0.05,vs normal cervical tissue) Conclusion: Progressively increasing expressions of DNMT1,DNMT 3A and 3B are associated with cervical carcinogenesis even during the precancerous stages.
出处
《泰山医学院学报》
CAS
2011年第5期321-323,共3页
Journal of Taishan Medical College
基金
国家自然科学基金资助项目(81060212)
内蒙古自然科学基金资助项目(2010BS1104)
中国博士后基金项目(20080430851)