宫颈癌组织中DNA甲基转移酶1、3A和3B mRNA的表达

Expression of DNMT1,3A and 3B mRNA in uterine cervical carcinoma tissue

目的探讨DNA甲基转移酶1、3A和3B在宫颈癌发生发展中的作用。方法应用real-time PCR技术检测10例宫颈癌、10例宫颈上皮内瘤变和10例正常宫颈组织DNMT1、3A和3BmRNA的表达。结果宫颈癌组织、宫颈上皮内瘤变和正常宫颈中DNMT 1mRNA对GAPDH mRNA的相对丰度值分别是0.30±0.1、0.26±0.12和0.10±0.04,宫颈癌组织组织中与正常宫颈中DNMT 1表达有显著差异(P<0.05);宫颈癌组织、宫颈上皮内瘤变和正常宫颈中DNMT3A mRNA对GAPDH mRNA的相对丰度值分别是0.94±0.27、0.51±0.24和0.13±0.04,三组相比较有显著性差异(P<0.05);宫颈癌组织、宫颈上皮内瘤变和正常宫颈中DNMT 3B mRNA对GAPDH mR-NA的相对丰度值分别是0.30±0.09、0.28±0.11和0.11±0.04,宫颈癌组织组织中与正常宫颈中DNMT 3B表达有显著差异(P<0.05)。结论 DNMT1、3A和3B参与了宫颈癌的发生,并在宫颈上皮内瘤变时就已经起了一定作用。

Objective： To study the relationship between DNMT1,3A and 3BmRNA expression and oncogenesis of uterine cervical carcinoma.Methods： The expressions of DNMT1,3A and 3B mRNA were detected by real-time PCR in 10 cases of cervical carcinoma,10 cases of cervical intraepithelial neoplasia（CIN） and 10 cases of normal cervical tissue.Results： The relative abundance of DNMT 1mRNA to GAPDH mRNA in cervical carcinoma,in CIN and in normal cervica were 0.30±0.1,0.26±0.12 and 0.10±0.04 respectively.The DNMT 1 mRNA was found to be increased in cervical carcinoma（P0.05,vs normal cervical tissue）.The relative abundance of DNMT 3A？mRNA to GAPDH mRNA in cervical carcinoma,in CIN and in normal cervica were 0.94±0.27,0.51±0.24 and 0.13±0.04,respectively.There were significant differences among three groups（P0.05）.The relative abundance of DNMT 3B mRNA to GAPDH mRNA in cervical carcinoma,in CIN and in normal cervica were 0.30±0.09,0.28±0.11 and 0.11±0.04 respectively.The DNMT 3B mRNA was found to be increased in cervical carcinoma（P0.05,vs normal cervical tissue） Conclusion： Progressively increasing expressions of DNMT1,DNMT 3A and 3B are associated with cervical carcinogenesis even during the precancerous stages.