银杏叶片对衰老大鼠海马CREB表达影响的实验研究

Effects of extract of Ginkgo Biloba on the expression of CREB in hippocampus of aging rats

目的研究银杏叶片对衰老大鼠海马CREB、pCREB表达的影响。方法雄性Wistar大鼠分为青年对照组(3月龄)、老年对照组(13月龄)和银杏叶组(13月龄)。银杏叶组大鼠每日灌胃银杏叶片,其余两组动物灌胃等量蒸馏水,共8w。Morris水迷宫实验观察大鼠空间记忆能力变化,Western Blot检测海马CREB、pCREB表达。结果 (1)与青年对照组相比,老年大鼠逃避潜伏期延长,穿越平台次数及平台象限停留时间减少(P<0.05);与老年对照组比较,银杏叶组大鼠逃避潜伏期缩短,穿越平台次数增多,平台象限停留时间延长(P<0.05)。(2)老年对照组大鼠海马CREB、pCREB表达水平低于青年对照组(P<0.05);与老年对照组相比,银杏叶组大鼠海马CREB、pCREB表达上升(P<0.05)。结论银杏叶片可改善老龄大鼠空间记忆功能,其机制可能与上调海马CA1区CREB、PCREB有关。

Objective To investigate effects of extract of Ginkgo biloba on expressions of CREB and pCREB in hippocampus of aging rats.Methods Male wistar rats were divided into three groups:young control group,old control group and EGb group.Rats in EGb group were treated with intragastric administration of EGb,while rats of the other two groups were treated with distilled water.The spatial learning and memory were evaluated by Morris water maze,and expressions of CREB and pCREB were detected by Western blot.Results Compared to young control group,the swimming time and times through platform in the target quardrant in old control group were reduced(P<0.05).Times passing through the platform and the time exploring the target quadrant were more and longer in EGb group than those of old control group(P<0.05).Compared with rats in young control group,relative contents of CREB and p-CREB in hippocampus were decreased in old control group(P<0.05).Relative contents of CREB and p-CREB were higher in EGb group than those in old control group(P<0.05).Conclusion EGb could ameliorate spatial learning and memory by increasing expressions of CREB and p-CREB in hippocampus of aging rats.