SB203580对受辐射小鼠免疫细胞的影响

The effects of SB203580 on the immune cells of irradiated mice

目的探讨p38抑制剂SB203580(SB)对辐射诱导免疫细胞的影响。方法将C57BL/6小鼠分为对照组、照射组、SB组。对照组接受假照射,其余2组进行2Gy全身照射。SB 15 mg/kg腹腔注射,照射24 h后开始给药,隔日1次,共给药5次。SB末次给药24 h后处死小鼠,取外周血进行分型CD4、CD8、B220检测,取骨髓测定细胞内活性氧(ROS)。结果照射组外周血白细胞,B220比例明显下降,CD4细胞比例及骨髓细胞ROS水平显著增加,与对照组比较差异显著(P<0.05)。SB组外周血淋巴细胞比例升高,骨髓ROS水平下降,与照射组相比差异显著(P<0.05)。结论 p38抑制剂对辐射引起的免疫细胞分型变化有一定的影响。

Purpose The aim of this study is to observe the effects of p38 inhibitor SB203580（SB）on the immune cells in the radiated mice.Methods C57BL/6 mice were divided into control group,irradiated group,and SB group.The control group received sham irradiation,and the rest accepted 2Gy total body irradiation,and dose rate 0.8 Gy/min.SB was intraperitoneally injected 15 mg/kg at 1,3,5,7,9 day after irradiation.After 2Gy irradiation 10 day,the peripheral bloods were collected by eyeballs enucleation.Then the immune cells phenotypes in peripheral bloods were analyzed by flowcytometry.Results The proportion of B220 and WBC in the irradiated mice significantly decreased,and CD4 and ROS levels of bone marrow cells increased significantly（P〈0.05）.Compared to irradiation group,lymphocyte rate increased and ROS levels of bone marrow cells decreased in the SB group（P〈0.05）.Conclusion The p38 inhibitor has a certain impact on the radiation-induced changes in immune cell phenotypes.