人参皂苷磷脂复合物的制备与质量评价初步研究

Preparation and preliminary study of quality evaluation of ginsenosides phospholipid compound

目的确定人参皂苷与磷脂形成复合物的最佳工艺,并进行质量评价.方法以人参皂苷与磷脂的复合率为评价指标,采用正交实验设计,考察反应溶剂类型、药-脂比、药物浓度、反应时间、反应温度等因素对磷脂复合物形成的影响.考察人参皂苷磷脂复合物的外观、紫外吸收特点.结果确定人参皂苷磷脂复合物的最佳制备条件:30℃,以四氢呋喃为反应溶剂,反应时间为1 h,药-脂比为1∶1,药物浓度为20mg·mL-1.在此条件下复合率为94.5%.结论磷脂复合物显著改善了人参皂苷的理化性质.

Objective To definite the optimal technology of ginsenosides phospholipids to form compound and carry out quality evaluation.Methods The binding rate of Ginsenosides and phospholipids as evaluation index,orthogonal design test was applied to inspect the effect of type of reaction solvent,drug-to-phospholipid ratio,drug concentration,reaction time and reaction temperature on the formation of phospholipid compound.The appearance and characteristic of ultraviolet absorption of ginsenosides phospholipid compound was investigated.Results The optimal conditions were obtained as follow：the reaction temperature was 30 ℃ with THF as reaction solvent;The reaction time was 1 h;The drug-to-phospholipid ratio was 1∶ 1 and the drug concentration was 20 mg·mL-1.The binding rate of Ginsenosides and phospholipids was 94.5%.Conclusion Phospholipid compound obviously improved the physico-chemical property of ginsenosides.