快速毛细管电泳安培法测定京尼平甘和藏红花素的含量

Determination of the Contents of Geniposide and Crocin in Fructus gardeniae by Capillary Electrophoresis

采用毛细管电泳安培法测定了栀子Fructus gardeniae中京尼平甘和藏红花素的含量。在20 mmol/L Tris-HCl缓冲溶液(pH=8.50),25 kV高压,0.86 V工作电极电位的条件下,在4 min内实现了两种被测组分的有效分离。测得四川栀子中京尼平甘和藏红花素的含量为32.46 mg/g和8.527 mg/g,浙江栀子中京尼平甘和藏红花素的含量为26.51 mg/g和7.028 mg/g。浙江栀子中京尼平甘和藏红花素的平均回收率分别为99.64%和100.2%,四川栀子中京尼平苷和藏红花素的平均回收率分别为99.73%和99.92%。该法快速准确,为栀子中京尼平甘和藏红花素的质量控制提供了新方法。

The capillary electrophoresis was used for the determination of the content of Geniposide and Crocin in Fructus gardeniae. The effective separated components were achieved by using separation voltage 25 kV, injection time 8 seconds and detection potential at 0. 86 V in 20 mmol/L Tris-HC1 buffer condition （ pH = 8.50）. The contents of Geniposide and Crocin in Sichuan Fructus gardeniae were determined to be 32.46 mg/g and 8. 527 mg/g. The contents of Geniposide and Crocin in Zhejiang Fructus gardeniae were determined to be 26.51 mg/g and 7. 028 mg/g. The average recovery ratios of Geniposide and Crocin in Sichuan Fructus gardeniae were 99.73% and 99.92%. The average recovery ratios of Geniposide and Crocin in Zhejiang Fructus gardeniae were 99.64 % and 100.2%. A novel method of the quality control of Geniposide and Crocin in Fructus gardeniae was built up.