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BTBD10蛋白在大鼠胰岛和INS-1细胞株的表达

Expression of BTBD10 protein in pancreatic islets and INS-1 cells of rats
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摘要 目的观察BTBD10蛋白在大鼠胰岛和INS-1细胞株的表达。方法利用构建好的BTBD10多克隆抗体,采用多重免疫荧光技术和激光共聚焦显微镜观察BTBD10在3月龄Fischer344大鼠的胰腺组织和大鼠胰岛β细胞株INS-1细胞中的表达。结果 BTBD10蛋白定位在大鼠胰腺组织的胰岛β细胞中,与胰岛素的定位重叠,而在胰腺的外分泌腺和胰岛α细胞中无表达,同时显示BTBD10定位在大鼠胰岛β株INS-1细胞的细胞浆中,表达量高。结论 BTBD10特异性表达于大鼠胰岛β细胞中,提示BTBD10可能与胰岛β细胞功能和胰岛素分泌有关。 Objective To observe the expression of BTBD10 protein in pancreatic islets and INS-1 cells of rats.Methods Expression of BTBD10 protein in pancreatic islets and INS-1 cells of Fischer344 rats at the age of 3 months was observed using BTBD10 polyclone antibody with multiple immunofluorescence staining techniques under laser confocal scan microscope.Results BTBD10 protein was expressed inβ-cells of rat pancreatic islets,but not in excrine glands and isletα-cells.Furthermore,BTBD10 and insulin were over-expressed in-cells and cytoplasm of INS-1 cells.Conclusion Specific expression of BTBD10 in β cells of rat pancreatic islets may be related with the function ofβcells and secretion of insulin.
作者 刘瑜 谷昭艳 李琳 李春霖 肖彧君 李剑 田慧
机构地区 解放军总医院老年内分泌科
出处 《军医进修学院学报》 CAS 2011年第10期1046-1047,1069,共3页 Academic Journal of Pla Postgraduate Medical School
基金 国家自然科学基金项目(30801201 30873412)~~
关键词 BTBD10 胰岛 INS-1细胞 BTBD10 islets of langerhans INS-1 cell
分类号 R341 [医药卫生—基础医学]
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参考文献7

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军医进修学院学报
2011年 第10期

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