摘要
目的对再生障碍贫血(aplastic anemia,AA)实验动物模型的免疫学相关指标进行检测和评估,为动物模型提供更全面的评估指标。方法建模BALB/C小鼠经γ射线照射后尾静脉注射DBA小鼠脾脏细胞,无菌饲养15日检测与再生障碍性贫血症相关的血常规和免疫学指标。结果 (1)模型组小鼠相比正常对照组,外周血常规指标指标与AA患者临床表现基本相符;(2)模型组小鼠外周血免疫学指标变化与临床AA患者基本相符。结论我们成功构建AA动物模型。
Objective Study on establishing aplastic anemia animal model and analyzing traditional and some immunologic examinations related to pathogenesis,to provide better platform for drugs development.Methods BABL/C mice were used to set up the model by using whole body γ-ray irradiation and caudal vein injection with spleen cells of DBA mice.After 15 days of experimental treatment,blood analysis,reticulocyte calculation,myelogram and some immunologic examination were performed.Results Mice of AA model group has marked reduction in the cells of three systems in peripheral blood,reticulocyte and marrow proliferation.The immunologic alteration of the AA model mice coincide with clinical clinical studies.Conclusion We successfully established AA animal model.Assessing immunologic alternation of the model based on clinical studies would provide us more issues to develop drugs.
出处
《中国实验诊断学》
北大核心
2011年第9期1421-1424,共4页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金(81072237)
北京协和医学院中国医学科学院放射医学研究所发展基金(SF0825)
