摘要
目的探讨左卡尼汀对急性心肌梗死模型大鼠梗死区TGF-β1表达的影响。方法将通过结扎冠状动脉左前降支48只心梗模型鼠随机分成3组:心梗对照组(MI-C)、盐水对照组(MI-N,1 ml/d)、左卡尼汀治疗组(MI-L,40 mg/kg.d)。另设假手术组(MI-Sham)。同步药物干预2周后,ELISA法检测AngⅡ水平、RT-PCR检测梗死区TGF-β1 mR-NA。结果血清中AngⅡ的浓度:与假手术组比较,心梗对照组、盐水对照组、左卡尼汀治疗组均明显升高(P<0.05)。左卡尼汀治疗组、盐水对照组与心梗对照组水平接近,差异无显著性(P>0.05)。梗死区TGF-β1 mRNA的表达:与心梗对照组比较,左卡尼汀治疗组表达下调,差异具显著性(P<0.05);生理盐水对照组与心梗对照组水平接近,差异无显著性(P>0.05)。结论左卡尼汀能够明显地减少急性心肌梗死大鼠梗死区TGF-β1的表达水平。
Objective To study the effect of Levocarnitine on the expression of transforming growth factor-β1（TGF-β1） mRNA in acute myocardial infarction（AMI） rat.Methods We randomized 48 rats surviving left anterior descending coronary artery ligation to placebo（MI-control）,isotonic saline solution（MIN,1ml/d） therapy and Levocarnitine（MI-L,40mg/kg.d） therapy with additional eight other rats being sham.Two weeks later,the level of plasma angiotensin Ⅱ（Ang Ⅱ） was detected using ELISA,and the expressions of TGF-β1 were measured by reverse transcription-polymerase chain reaction（RT-PCR）.Results Plasma Ang Ⅱ concentration evidently raised in the MI-control rats,MI-saline rats and MI-Levocarnitine rats,compared with MI-sham rats.There was no significant difference between MI-saline rats and MI-Levocarnitine rats.The expressions of TGF-β1 were as follow： Compared with sham rats,the expressions of TGF-β1 of MI-control rats and the other two admission groups were significantly increased（P〈0.05）.Compared with MI-control rats,the proteins were down-regulated in MI-Levocarnitine rats.There were no significant differences between MI-control rats and MI-saline rats（P〉0.05）.Conclusion The Levocarnitine can significantly inhibit the production of TGF-β1 in case of non-interference of angiotensin.
出处
《中国实验诊断学》
北大核心
2011年第9期1444-1446,共3页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金资助项目(30770883)
