大鼠尿液中壬基酚的代谢轮廓

Metabolic Profiles of Nonylphenol in Rat Urine

运用代谢组学方法研究了壬基酚对大鼠尿液代谢的影响,通过高效液相色谱-飞行时间质谱技术建立了大鼠尿样的代谢指纹图谱,用主成分分析法分析给药组与对照组代谢物指纹图谱的差异,通过t检验选取潜在的生物标志物及效应标志物,并结合代谢物数据库检索对其进行鉴定.结果表明,壬基酚给药后,尿液中含量变化显著的成分苯基葡萄糖苷酸、L-高半胱氨酸、3-硝基丙酸、肌酸酐、左旋肉碱及5-羟色胺等构成了大鼠尿液代谢的轮廓,它们在生物体内的变化可能对生殖系统、免疫系统、神经系统及脂肪的代谢造成一定的影响,从代谢的角度解释了环境雌激素壬基酚对生物体的危害,为毒理学研究提供了依据.

The metabonomics method was applied to investigate the effects of nonylphenol（NP） on urine metabolism in Sprague Dawley rat.The profiles of urine samples were obtained with high performance liquid chromatography coupled with time-of-flight mass spectrometry（HPLC/TOF-MS）.The data were analyzed with the principle components analysis（PCA） to distinguish the differences of profiles between the NP groups and the control group.The potential biomarkers and effective biomarkers were screened out according to the t-test,and identified with Metlin Scripps Center for mass spectrometry database.The results indicated that the contents of phenyl glucuronide,homocystein,3-nitropropionic acid,creatinine and 5-hydroxytryptamine changed obviously in urine after exposure to NP,which constituted the metabolic profiles in rat urine of NP,and might injury reproductive system,immune system,nervous system and lipometabolism in metabolism process.This study provides the basis for toxicology studies on NP to organisms from the standpoint of metabolism.