气相色谱-质谱法同时测定氧化型染发剂中的17种染料

Simutaneous Determination of 17 Components in Oxidative Hair Dyes by Gas Chromatography-Mass Spectrometry

建立了氧化型染发剂中17种染料中间体(包括苯二胺类、氨基酚类、苯二酚类、萘酚和氨基吡啶类等)的气相色谱-质谱检测方法.采用体积分数为50%的乙醇超声提取染发剂中的染料中间体,加入还原剂连二亚硫酸钠防止待测物变质,选择DB-WAX毛细管柱实现了在25 min内同时分离17种水溶性和醇溶性染料.结果表明,本方法对所测物质的检出限(LOD)为2～380 mg/kg,定量限(LOQ)为7.5～1267.5 mg/kg.在1,2,10,20或100倍LOQ 4个添加水平下的平均回收率在81.1%～103.7%之间,相对标准偏差(n=6)在0.67%～3.73%之间.

A reliable Gas Chromatography-Mass Spectrometry（GC-MS） method was developed for the simultaneous determination of 17 dye intermediates,including benzenediamines,aminophenols,benzenediols,naphthalenediol and diaminopyridine,in oxidative hair dyes.Samples were extracted with 50% ethanol by adding sodium dithionite to prevent oxidation.A DB-WAX capillary column was selected for the separation of both water soluble and alcohol soluble hair dye intermediates.The results showed that 17 dye intermediates were well separated within 25 min.The detection limits of these compounds were in the range of 2—380 mg/kg.The recoveries of the target compounds in hair dyes ranged from 81.1% to 103.7% with four addition levels.The method described was validated by 5 different laboratories and successfully applied to the analysis of commercial oxidative hair dyes.