血红蛋白和SiO_2凝胶层层组装膜在碳纳米管/金纳米粒子修饰电极界面上的直接电化学及电催化研究

Electrochemistry and Electrocatalysis of Hemoglobin with SiO_2 Gel Film Layer-by-layer Assembled on Carbon Nanotubes-Au Nanoparticles Modified Electrode

以SiO2凝胶膜和蛋白质交互组装法固定血红蛋白(Hb),对其进行了电化学和电催化研究.首先制备碳纳米管/金纳米粒子复合材料修饰的MWNTs-Au/GC电极,为防止蛋白质在电极表面流失,将Hb和自制的SiO2凝胶膜交替滴涂到电极表面,得到SiO2/Hb层层组装膜修饰电极,即{SiO2/Hb}n/MWNTs-Au/GC电极,n=2为优化层数.Hb在{SiO2/Hb}2/MWNTs-Au/GC电极上仍能保持其特有的生物活性,并能与电极进行稳定快速的电子直接转移,同时表现出过氧化物酶特性,对H2O2具有良好的生物电催化还原能力.

Protein direct electrochemistry study and its electro-catalysis analysis were introduced,involving layer-by-layer alternate assembly of hemoglobin（Hb）and SiO2 gel film onto electrode surface.The electrode was firstly modified with the nano-compound of multi-walled carbon nanotubes and Au nanoparticles,i.e.,MWNTs-Au/GC electrode.Then Hb and SiO2 gel film were alternately spread onto the electrode surface to stabilize the protein and its amount and bioactivity,thus resulting {SiO2/Hb}n/MWNTs-Au/GC electrode.Under the optimized n=2,the Hb on the {SiO2/Hb}2/MWNTs-Au/GC electrode displayed its intrinsic bioactivity well,transferring electrodes with electrode and catalyzing H2O2 reduction as one peroxidaze enzyme.