糖尿病小鼠小肠黏膜扫描电镜的观察

Observation of small intestinal mucosa of diabetic mice by scanning electron microscope

目的观察糖尿病小鼠小肠黏膜吸收通路的形态学是否改变,为进一步研究糖尿病的消化系统并发症提供形态学基础。方法分别选取3、5、6、8、10月龄db/db糖尿病小鼠及相应年龄段的db/+m正常小鼠,每组6只。灌流固定后用扫描电镜观察小肠黏膜上皮及上皮下固有层的结构。结果小肠绒毛比对照组宽大;上皮细胞侧面上部的微皱褶稀疏,排列不规则;绒毛中轴的筛状孔比对照组多。结论糖尿病小鼠小肠黏膜的形态发生了改变。糖尿病时小肠黏膜的形态变化有利于物质的异常吸收。

Objective To observe if the shape of absorbing route in small intestinal mucosa of diabetic mice was changed,and to provide basement of morphology for the next research of complication in digestive system.Methods 3、5、6、8、10 month-age db/db mice were used in test groups,and age-matched db/＋m mice were used as control group.Each group included 6 mice.Samples of intestines were obtained after being infused to observe epithelial cell and structure of below epithelium.Results In the diabetic group,intestinal villus was larger than that in control group,tiny creases in lateral cell membranes on the upper parts became rare and irregular,and the quantity of cribriform holes were more than that in control group.Conclusion The shape of small intestinal mucosa in diabetic mice was changed.