金属框架结构材料MOF-199对漆酶的固定化及其性质

Immobilized laccase and its properties by metal organic framework MOF-199

以金属框架结构材料MOF-199为载体对漆酶进行固定化,并对固定化酶的性质进行初步研究。首先,以3-氨基丙基三乙氧基硅烷对载体MOF-199进行表面氨基化修饰,再用戊二醛对载体进行活化,最后对漆酶进行固定化。固定化条件优化结果表明:在漆酶质量浓度0.3 g/L,戊二醛用量1%(体积分数),pH 4.8下固定7 h,制得固定化酶活性最高。对固定化酶的研究发现:最适反应温度为40℃,最适pH为5.2,在连续操作7次后,固定化酶的活力仍能保持在51%。固定化漆酶热稳定性,pH耐受性,贮存稳定性均明显高于游离漆酶。

The immobilization of laccase and the properties of the immobilization laccase were studied. Laccase was immobilized by metal-organic framework MOF-199 as the carrier and 3-aminopropyltriethoxysilane was modified on the surface of the carrier, then glutaraldehyde as crosslinking agent. The laccase immobilized on MOF-199 were optimized to achieve the most optimal conditions of immobilization. The results showed that： laeease concentration was 0.3 g/L, load of glutaraldehyde was 1% （ V/V）, the value of pH is 4. 8, and the cross-linking time is 7 h. Results showed that the immobilized laccase optimum temperature is 40 ℃, the optimum pH is 5.2, after 7 times in continuous operation, the enzyme activity still remained at 51%, the thermal stability of immobilized laccase, pH tolerance, storage stability were higher than that of the free laccase.