新疆棉花秸秆中乳酸菌的分离与鉴定

Isolation and identification of lactic acid bacteria from cotton stalk in Xinjiang

实验用MRS+CaCO3固体培养基,以新疆吐鲁番地区棉花秸秆为实验对象,分离纯化得到乳酸菌4株。经形态学、生理生化特性和16S rRNA序列同源性分析进行鉴定,结果表明:Z1、Z2菌株为植物乳杆菌(Lactobacillus plantarum),Z4、Z5菌株为戊糖片球菌(pediococcus pentosaceus)。同时测定已鉴定的4株乳酸菌的产酸速率,结果发现,Z2和Z5表现出较强的产酸速率,可进一步研发成青贮饲料添加剂。

We used MRS＋CaCO3 solid medium to isolated 4 strains of lactic acid bacteria from Cotton stak sample which was gathered from in Xinjiang.According to the morphological characters,physiological and biochemical properties,16S rRNA gene sequence determination method.The results showed that Z1,Z2 strains of Lactobacillus plantarum,Z4,Z5 strains of pediococcus pentosaceus.The acid-production rate of the 4 strains was determined respectively,then found that the acid-production rate of Z2and Z5 were faster,which can be developed into silage additives.