羟基红花黄色素A注射液调节血脂作用初探

The primary research on hydroxyl safflor yellow A injection mediating blood lipid

目的观察羟基红花黄色素A调节血脂的作用，并探讨其初步机理。方法将50只小鼠按体重随机分成正常动物组（A组）、高脂血症模型组（B组）、羟基红花黄色素A注射液低、中、高三种剂量组（C、D、E组）。C、D、E组每天分别注射10、40、70mg／kg羟基红花黄色素；A、B组每天分别注射生理盐水0．4ml。连续注射3d后，对B～E组分别造模，18h后测定所有组别胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL-C）、高密度脂蛋白（HDL-C）、丙二醛（MDA）的含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH—Px）的活性。结果成模高脂血症小鼠静脉注射羟基红花黄色素高剂量组，与给药前比较，血清中TC（4．09±0．2）mmol／L、TG（0．96±0．15）mmol／L、LDL-C（5．87±0．17）mmol／L、HDL．C（0．83±0．21）mmol／L、MDA（8．26±1．05）nmol／ml水平和SOD活性（330．18±11．45）U／ml、GSH．Px活性（1023．54±25．34）U／ml均有明显变化，差异均有统计学意义（P均〈0．05）。结论羟基红花黄色素A具有调节血脂的作用，且可能是通过抗氧化作用调节血脂。

Objective To investigate the effect of hydroxyl safflor yellow A on mediating blood lipid and to explore its primary mechanism. Methods Fifty KM mice were divi（ted into five groups randomly： control group （A）, hyperlipidemia model group （B）, high-dose group （C）, mid-dose group （D）and low-dose group（E）. C, D and E group were injected by hydroxy safflor yellow A with 10, 40 and 70 mg·kg-1 day-1 respectively, while A and B group were both injected by saline with 0.4 ml day-1, the administrations were kept on three days. The levels of serum total cholesterol（TC）, triglycerides（TG）, low-density lipoprotein cholesterol （LDL-C）, high-density lipoprotein cholesterol （HDL-C） and malondialdehyde （MDA） were assayed, simultaneously the activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） were assayed after 18 hours. Results Compared with the control group, the serum TC （4.09± 0.2） mmol/L, TG （0.96 ± 0.15） mmol/L, LDL-C （5.87±0.17） mmol/L, HDL-C （0.83±0.21） mmol/L, MDA （8.26±1.05） nmol/ml, and the activities of SOD （330.18± 11.45 ） U/ml, GSH-Px （ 1023.5± 25.34 ） U/ml of model group injected high doses of hydroxysafflor yellow A were statistically significant. Conclusion Hydroxy safflor yellow A had the function of mediating blood lipid. Anti-oxidation could be the mechanism.