前列腺增生对老年大鼠膀胱胶原沉积的影响

Effect of Benign Prostatic Hyperplasia on the Deposit of Collagen on the Ageing Rat Bladder

目的:观察老年大鼠膀胱形态学的改变以及BPH对其影响,探讨BPH对老年大鼠膀胱形态及胶原沉积的影响。材料与方法:材料:青年(3月龄)及老年(18月龄)雄性Wistar大鼠。除空白青年组及空白老年组外,其它大鼠手术去势加皮下注射睾酮法复制BPH模型。将模型大鼠按月龄随机分为模型青年组、模型老年组、模型老年组+非那雄胺组、模型老年组+多沙唑嗪组。各组大鼠分别给予相应药物灌胃30天。免疫组织化学染色法观察膀胱组织胶原I及胶原Ⅲ含量。结果:老年大鼠膀胱逼尿肌细胞明显减少,排列稀疏,间质中胶原组织沉积尤为明显。BPH老年大鼠的膀胱逼尿肌细胞较正常青年、老年大鼠均明显增加,经多沙唑嗪和非那雄胺治疗后,BPH老年大鼠的膀胱逼尿肌细胞数量有所下降。空白老年组膀胱组织中胶原I阳性面积(18.16±3.89)比率高于空白青年组(9.74±1.54,P<0.01),低于模型老年(23.30±1.32,P<0.01)、多沙唑嗪(23.37±2.94,P<0.01)、非那雄胺(21.94±3.23,P<0.05)等各组。模型老年组膀胱组织中胶原Ⅲ阳性面积比率(40.11±2.03)明显高于空白老年组(26.49±3.12,P<0.01)、多沙唑嗪(30.88±4.73,P<0.01)和非那雄胺组(26.13±2.46,P<0.01)。空白老年组膀胱组织胶原Ⅲ/胶原I(0.68±0.10)明显低于空白青年组(1.30±0.28,P<0.01)。多沙唑嗪(0.78±0.18,P<0.05)、非那雄胺组(0.82±0.09,P<0.01)明显高于模型老年组(0.58±0.36),但与空白老年组相比没有显著性差异。结论:老年膀胱改变是老年前列腺增生大鼠膀胱改变的重要原因,单纯解除梗阻不能完全改善之。这一结果提示我们在临床上要充分考虑前列腺增生患者膀胱自身的衰老改变,及早保护膀胱功能以延缓其衰老改变的过程。

Objective：To observe the morphologic changement of aged bladder and the effect of benign prostatic hyperplasia（BPH） on the deposit of collagen on the ageing rat bladder.Materials and Methods：Materials：Young（3 months） and aged（18 months） male Wistar rats.In addition to blank youth group and blank elderly group,the other rats were injected testosterone plus surgical castration replicated with BPH model.The model rats were randomly divided into model months old youth,older model group,model group ＋ elderly finasteride group,model elderly group ＋ doxazosin group.The rats were fed for 30 days to give the corresponding drugs.Immunohistochemical staining of bladder tissue with collagen Ⅲ and collagen I.Results：The aged rats significantly decreased detrusor cells,and increased collagen deposition.Detrusor smooth muscle cells in old BPH rats were significantly increased compared with normal detrusor young and old rats,and decreased after treatment by doxazosin and finasteride.Collagen I positive area ratio（18.16±3.89） were higher than that of young group（9.74±1.54,P0.01）,lower than older model group（23.30±1.32,P0.01）,doxazosin group（23.37±2.94,P0.01）,and finasteride group（21.94±3.23,P0.05）.collagen Ⅲ positive area ratio of old model group bladder（40.11±2.03） was significantly higher than blank elderly（26.49±3.12,P0.01）,doxazosin group（30.88±4.73,P0.01） and finasteride group（26.13±2.46,P0.01）.The rate blank elderly group（0.68±0.10） was significantly lower than blank young group（1.30±0.28,P0.01）.The rate of collagen Ⅲ/collagen I of Doxazosin group（0.78±0.18,P0.05）and finasteride group（0.82±0.09,P0.01） was significantly higher than the model group（0.58±0.36）,but there was no significant differences compared with blank elderly group.Conclusion： The aged change is an important reason for changes in aged BPH rat bladder,simply remove the obstruction cannot improve it.The results suggest that we should give full consideration on the aging changes in the bladder itself in clinical patients with benign prostatic hyperplasia,it is important of early protection of bladder function in order to delay the aging process of change.