荧光原位杂交技术在膀胱尿路上皮肿瘤病理学诊断中的初步应用

Preliminary Application of Fluorescence in Situ Hybridization in Pathological Diagnosis of Bladder Urothelial Tumor

目的应用荧光原位杂交(fluorescence in situ hybridization,FISH)技术,了解膀胱尿路上皮肿瘤细胞核染色体畸变情况及其对病理诊断及鉴别诊断的应用价值。方法采用3、7、17号染色体着丝粒及p16基因探针,对33例不同级别膀胱尿路上皮肿瘤组织和10例正常对照膀胱组织进行FISH检测。结果 3、7、17染色体及p16扩增率与缺失率,浸润性尿路上皮癌与其他5组比较均有统计学意义(P<0.01);而其他各组间比较无统计学意义(P>0.05),4种探针联合检测≥2个指标出现异常,浸润性尿路上皮癌占100%,与其他5组比较有统计学意义(P<0.01);该指标对浸润性尿路上皮癌诊断敏感性为81.82%、特异性为91.67%。结论应用FISH技术,可以了解膀胱尿路上皮肿瘤组织3、7、17染色体及p16基因的畸变情况,FISH技术还可作为尿路上皮肿瘤病理学诊断与鉴别诊断及术后监测的重要手段。

Objective To investigate the value of fluorescence in situ hybridization（FISH）for detecting the chromosome aberration and diagnosis and differential diagnosis of urothelial tumors.Methods 3,7,17 chromosome and p16 gene were analyseed by FISH in 33 urothelial tumor specimens and 10 normal bladder tissues.Results The positive rate of 3,7,17 chromosome and p16 genes amplification and p16 gene deletion in invasive urothelial cancer were significantly higher than other 5 groups of urothelial tumors（P〈0.01）.All of invasive urothelial carcinoma showed more than two abnormal indexes Sensitivity and specificity of more than two abnormalindexes in diagnosis of invasive urothelial cancer was 81.82% and 91.67% respectively.Conclusion FISH can be used for analyzing 3,7,17 chromosome and p16 gene amplification and diagnosis/differential diagnosis in urothelial tumors.