雌激素受体a基因启动子甲基化在卵巢上皮癌中表达的意义

Expression of the Methylation of ERa Gene Promotor in Ovarian Cancer

目的探讨卵巢上皮癌ERa启动子甲基化与其临床病理特征的关系。方法选取10例正常卵巢组织、20例卵巢良性肿瘤组织以及64例卵巢上皮癌组织,应用甲基化特异性PCR(Methylation Specific PCR,MSP)及测序法,分别检测ERa基因启动子区CpG岛的甲基化状态。结果 64例卵巢癌组织中,ERa基因启动子甲基化率为53.13%,20例卵巢良性肿瘤和正常卵巢组织中ERa甲基化率分别为10.00%和0。卵巢癌组ERa启动子甲基化率较良性组和正常组均显著增高(P<0.05)。ERa启动子甲基化与卵巢癌病理分期和病理分级呈显著负相关性。结论卵巢癌组织中ERa基因表达沉默与其启动子CpG岛甲基化密切相关,后者有可能成为卵巢癌预后不良的分子检测指标。

Objective To study the relationship between ERa gene promotor methylation and the clinicopathological characteristics in ovarian cancer.Methods MSP method was used to detect ER a gene expression in 10 normal ovarion tissues,20 benign ovarian tissues and 64 cases of ovarian cancer tissues,The methylation of CpG of gene promoter was respectively detected using PCR sequencing.Results In 64 cases of ovarion cancer tissues,the methylation of ER a promotor were 53.13%;while in 10 normal cases and 20 benign tissues the methylatlon rates of ERa is 10.00% and 0 respectively（P〈0.05）.The methylation rates of ERa in ovarian cancer were significantly higher than that in normal and benign tissues（P〈0.05）.There was a negative relation between gene methylayion and clinicopathological characteristics in ovarian cancer.Conclusion Silencing of ERa gene expression is suggested to be significantly related with the methylation CpG island in the ERa promotor in ovarion cancer.The methylation of CpG island may be molecular marker indicating bad prognosis of ovarion cancer.