塞来昔布对宫颈癌细胞的化疗增敏作用

Effect of Celecoxib on Chemotherapy Sensitization for Cervical Cancer Cells

目的探讨特异性环氧化酶-2(COX-2)抑制剂塞来昔布对顺铂诱导宫颈癌Hela细胞增殖与凋亡的作用及机制。方法采用四甲基偶氮唑蓝比色法(MTT),测定塞来昔布对顺铂诱导Hela细胞增殖活性的作用;应用流式细胞仪检测细胞凋亡率;Western blot检测Hela细胞中Bcl-2、Bax蛋白的表达情况。结果 5、10μmol/L的塞来昔布分别与顺铂联合应用,可增强顺铂对Hela细胞增殖抑制作用。塞来昔布(5μmol/L)能促进顺铂诱导Hela细胞的凋亡。塞来昔布作用于Hela细胞后,Bcl-2蛋白表达水平下调、Bax蛋白表达水平上调呈浓度依赖关系。结论塞来昔布能促进顺铂对Hela细胞增殖抑制和诱导凋亡作用,起化疗增敏作用,可能与其下调Bcl-2蛋白表达水平、上调Bax蛋白表达水平有关。

Objective To investigate the effect and its mechanism of celecoxib on cisplatin-induced proliferation and apoptosis in cervical cancer Hela cells.Methods The proliferation of celecoxib on cisplatin-induced cervical cancer Hela cells was examined by MTT assay.The apoptosis of Hela cells were tested by PI staining flow cytometry.The expression and their activity change of Bcl-2,Bax protein in Hela cells were investigated by Western blot.Results 5,10μmol/L concentrations of celecoxib combined with cisplatin significantly increased the cytotoxicity of cisplatin to Hela cells.Celecoxib（5μmol /L） significantly promoted cisplatin to induced Hela cells apoptosis.the expression levels of Bcl-2 protein were decreased and the expression level of Bax protein was increased in Hela cells treated with celecoxib in a concentration dependent manner（P〈0.05）.Conclusion Celecoxib could enhance the effect of growth inhibtion and apoptosis induction of cisplatin on Hela cells.The effect of chemosensitization to cisplatin of celecoxib on Hela cells may be associated with downregulating the expression of Bcl-2 protein and upregulating the expression of Bax protein.