正交试验法优选犬问荆山奈素提取中水解条件的研究

Study on Optimal Hydrolysis Condition of Kaempferol Extraction From Equisetum Palustre

目的:优选犬问荆山奈素提取中的水解条件。方法:以山奈素含量为指标成分,采用正交试验法,考察水解时间、水解温度和加盐酸量3个因素(每个因素取3个水平)对山奈素提取的水解条件进行优选。结果:山奈素提取的最佳水解条件为加盐酸2.5 mL、60℃水解30 min。结论:优选得到山奈素提取的水解条件稳定、合理。

Objective：To optimize hydrolysis condition of kaempferol extraction from Equisetum palustre.Method：Orthogonal design was adopted and content of kaempferol was taken as index.The volume of hydrochloric acid,time and temperature of hydrolysis were selected as variable factors.Thus,the optimum condition was determined.Results：The optimum hydrolysis condition for kaempferol were as follows：hydrolyzing for 30 min at 60 ℃ with 2.5 mL hydrochloric acid.Conclusion：The optimized hydrolysis condition is stable and feasible.