落葵果实中总黄酮提取条件的研究

Study on the Extraction Conditions of Total Flavonoids from Fruit of Basella rubra L.

采用超声波提取法,通过正交实验确定从落葵果实中提取总黄酮的最佳工艺条件,并用比色法测定提取物中总黄酮的含量.结果表明,最佳条件是以体积分数为70%的乙醇为提取剂、料液比为1:20、提取时间为35min.在此条件下,落葵果实总黄酮提取量为5.3 mg蛐g.

The orthogonal experiment to screen out the optimal extraction conditions of total flavonoids from fruit of Basella rubra L.by ultrasonic extraction was utilized.The total flavonoids content extracted from fruit of Basella rubra L.was determined by spectrophotometry.The results showed that the optimal extraction conditions： Extractant is 70% ethanol,solid to liquid ratio is 1：20,the extraction time is 35 minutes.Under the extraction conditions,the yield of total flavonoids from fruit of Basella rubra L.was 5.3 mg.g-1.