摘要
为提高酒精酵母细胞发酵液中海藻糖的含量,在制备出透性化酒精酵母细胞的基础上,用单因素实验和响应面分析法,优化了酒精酵母的发酵培养基,确定了最适培养基组成。实验结果表明,酵母膏、蔗糖及氯化钠的添加量对海藻糖积累影响显著,影响程度依次为:酵母膏>蔗糖>氯化钠。优化后的培养基组成为:酵母膏14.7g/L,蔗糖32.5g/L,氯化钠27.4g/L,此时海藻糖产量达到0.931 9g/L。
In order to enhance the trehalose accumulation in permeabilized alcohol yeast, single-factor test and response surface methodology were used to optimize the fermentation medium. All tested factors have significant effect on the trehalose accumulation as following order: yeast extract sucrose )sodium chloride. The optimal fermentation medium was obtained as sucrose concentration 32.5 g/L, level of yeast extract 14.7 g/L, sodium chloride concentration 27.4 g/L in which the yield of trehalose reached 0. 931 9 g/L.
出处
《大连工业大学学报》
CAS
北大核心
2011年第5期329-332,共4页
Journal of Dalian Polytechnic University
基金
辽宁省教育厅科学技术研究项目(2020701)
关键词
酒精酵母
海藻糖
透性化
alcohol yeast
trehalose
permeabilized