透性化酒精酵母细胞产海藻糖发酵培养基的优化

Optimization of fermentation medium for trehalose production by permeabilized alcohol yeast cells

为提高酒精酵母细胞发酵液中海藻糖的含量,在制备出透性化酒精酵母细胞的基础上,用单因素实验和响应面分析法,优化了酒精酵母的发酵培养基,确定了最适培养基组成。实验结果表明,酵母膏、蔗糖及氯化钠的添加量对海藻糖积累影响显著,影响程度依次为:酵母膏>蔗糖>氯化钠。优化后的培养基组成为:酵母膏14.7g/L,蔗糖32.5g/L,氯化钠27.4g/L,此时海藻糖产量达到0.931 9g/L。

In order to enhance the trehalose accumulation in permeabilized alcohol yeast, single-factor test and response surface methodology were used to optimize the fermentation medium. All tested factors have significant effect on the trehalose accumulation as following order： yeast extract sucrose ）sodium chloride. The optimal fermentation medium was obtained as sucrose concentration 32.5 g/L, level of yeast extract 14.7 g/L, sodium chloride concentration 27.4 g/L in which the yield of trehalose reached 0. 931 9 g/L.