四氨基酞菁锌负载二氧化硅纳米粒子的合成及其对细胞毒性的研究

Preparation of Tetraamino-Phthalocyanine Zinc Loaded Silica Nanoparticles and Its Cytotoxicity Study

通过在无极核微乳液中水解乙烯基三乙氧基硅烷(TEVS)和3-氨丙基三乙氧基硅烷(APTES),制备了疏水性光敏剂-2,9,16,23-四氨基酞菁锌负载的表面带有正电荷的二氧化硅纳米粒子(SiO2@ZnPc(NH2)4)。通过透射电镜(TEM)、Zetasizer Nano-ZS粒度仪(DLS)、紫外-可见分光光度计(UV-Vis)研究和表征了该纳米粒子的表面形貌、表面电荷、水溶性和稳定性。所合成的纳米粒子粒径约20nm,颗粒为规则的球形,粒径较均一,具有很好的分散性,平均ζ电位值为(28.8±2.79mV),在714nm处有强吸收峰。此外,负载在纳米粒子中的ZnPc(NH2)4不易泄漏,增强了ZnPc(NH2)4的抗光漂白能力。以1,3-二苯基异苯并呋喃(DPBF)为探针分子,检测出SiO2@ZnPc(NH2)4可以有效产生单线态氧。通过测量纳米粒子与活细胞(HeLa,U251,PC-12)孵育后的细胞活度来检测对细胞的毒性,结果显示,纳米粒子浓度≤300mg/L时,未掺杂ZnPc(NH2)4的二氧化硅纳米粒子(SiO2-NH2)对细胞没有毒性;当其负载ZnPc(NH2)4后,对细胞没有显著毒性。

Positive charged silica nanoparticles,entrapping hydrophobic photosensitizer 2,9,16,23-tetraamino-phthalocyanine zinc（ZnPc（NH2）4）,have been synthesized in the nonpolar core of micelles by hydrolysis of triethoxyvinylsilane（TEVS） and 3-aminopropyltriethoxysilane（APTES）.The water-soluble,stability,surface morphologies and charge were characterized using transmission electron microscopy（TEM）,dynamic light scattering（DLS） and UV-visible spectrophotometer.The as-prepared nanoparticles are highly monodispersed spheres with uniform diameter（about 20 nm）,and stable in aqueous system.Its average ζ potential value is 28.8±2.79 mV and exhibits strong Absorption peak at 714 nm.In addition,encapsulation of ZnPc（NH2）4 in the silica nanoparticles prevented ZnPc（NH2）4 from leaking and enhanced the anti-photobleaching.The ZnPc（NH2）4 entrapped-nanoparticles（SiO2@ZnPc（NH2）4） can efficiently generat singlet oxygen as measured by chemical probe 1,3-diphenylisobenzofuran（DPBF）.The toxicity of silica nanoparticles to cells has been investigated by the incubation of the ZnPc（NH2）4 entrapped-nanoparticles and the nanoparticles without ZnPc（NH2）4（SiO2-NH2） with living cancer cells（HeLa,U251,PC-12）.The experimental result reveals that both SiO2@ZnPc（NH2）4 and SiO2-NH2 particles have no significant toxicity when the concentration of the particles is below 300 mg/L.