VDR基因TaqⅠ位点单核苷酸多态性与宁夏回汉人群中重度慢性牙周炎易感性的相关性

The association between VDR gene TaqⅠ SNPs and moderate to severe chronic periodontitis in Ningxia population

目的探讨维生素D受体基因(VDR)TaqⅠ位点单核苷酸多态性(SNP)与宁夏回汉人群中重度慢性牙周炎(CP)之间的相关性,并分析VDR基因的基因型和等位基因频率在回、汉人群中的分布是否有差异。方法收集中重度CP患者178例(汉族90例、回族88例)和健康对照者187例(汉族95例、回族92例)的静脉血,采用PCR-RFLP方法测定TaqⅠ位点的基因型,应用病例对照研究分析VDR基因SNP与中重度CP的易感性是否有相关关系。结果回族CP组和回族健康对照组在基因型的分布上有显著性差异,等位基因频率的分布上亦有显著性差异(P值分别为0.037,0.043);汉族CP组和汉族健康对照组在基因型的分布上有显著性差异,等位基因频率的分布上亦有显著性差异(P值分别为0.033,0.039);汉族CP组和回族CP组比较,两组在基因型和等位基因频率的分布上均无显著性差异(P值分别为0.785,0.788)。结论 VDR基因TaqⅠ(rs731236)位点多态性与回族中重度CP和汉族中重度CP的易感性均可能存在一定的相关性。

Objective To determine whether moderate to severe chronic periodontitis（CP） in Ningxia population was associated with polymorphisms in vitamin D receptor（VDR） gene（TaqⅠ）,and to analyze if there were differences in the frequencies of VDR genotypes and alleles between Han nationality and Hui nationality.Methods Peripheral venous blood from participants was collected,including 90 CP patients and 95 healthy controls in Han nationality.88 CP patients and 92 healthy controls in Hui nationality were selected.Whether there were associations between VDR gene polymorphisms and CP by case-control methods was analyzed.Results There was statistically significant difference in the frequencies of VDR TaqⅠ genotypes and alleles between the CP of Hui nationality and healthy controls（P = 0.037 and P = 0.043,respectively）,CP patients and healthy controls in Han nationality（P = 0.033 and P = 0.039,respectively）.There was no statistically significant difference in the frequencies of VDR TaqⅠ genotypes and alleles between the CP in Han nationality and CP in Hui nationality（P = 0.785 and P = 0.788,respectively）.Conclusions The TaqⅠ polymorphism of the VDR gene was associated with the moderate to severe CP in Hui and Han nationality.