慢性肺动脉高压大鼠上腔静脉血流流速曲线的变化特征分析

Characteristics of flow velocity waveform of the superior vena cana in rat model with chronic pulmonary hypertension

目的探讨野百合碱所致慢性肺动脉高压大鼠上腔静脉血流流速曲线的变化特征及其意义。方法雄性成年SD大鼠40只，其中实验组32只，采用腹腔注射野百合碱溶液建立慢性肺动脉高压模型；对照组8只，注射相同剂量的乙醇生理盐水混合液。28d后行右心导管测量肺动脉压力。实验组干预前及干预后28d经锁骨上窝切面对上腔静脉进行多普勒超声检测。结果成功建模29只，实验组肺动脉收缩压高于对照组（P〈0．001）。上腔静脉血流流速曲线显示实验组干预后28d双侧上腔静脉离心波峰值血流速度（VAR）、VAR与向心波峰值血流速度（Vs）比值升高，与干预前比较差异有统计学意义（P〈0．01）；右上腔静脉Vs较干预前降低，差异有统计学意义（P〈0．05）。结论慢性肺动脉高压大鼠双侧上腔静脉VAR、VAR／Vs值较干预前明显升高，肺动脉高压时上腔静脉血流流速曲线的变化具有特征性。

Objective To explore the characteristics of the flow velocity waveform of superior vena cana （ SVC ） in a rat model with chronic monocrotaline-induced pulmonary hypertension by echocardiography. Methods The chronic pulmonary hypertension model was developed by monocrotaline solution （60 mg/kg） injection into the abdominal cavity in 32 SD rats. Eight rats with the placebo injected served as controls. An examination of the flow velocity waveform of SVC was performed by Doppler echocardiography via supraclavicular fossa before and 28-day after the injection. The pulmonary arterial systolic pressure was measured by pressure-guided micro-catheter 28-day after the injection both in the control and the pulmonary hypertension model group. Results Chronic monocrotaline-induced pulmonary hypertension was successfully developed in 29 rats. The flow velocity waveform of SVC showed that the ratio of the maximum reverse peak flow velocity due to atrial contraction （VAR） to the maximum systolic peak flow velocity （Vs） and VAR increased dramatically 28-day after the monocrotaline solution injection in both sides of SVC （ P 〈0.01）,while only the Vs of the right SVC significantly decreased （ P 〈0.05）. Conclusions The changes of VAR/Vs ratio and VAR in rat model with chronic monocrotaline-induced pulmonary hypertension suggested that the changes of flow velocity waveform of SVC have indicating features in reflecting pulmonary hypertension.