妊娠高血压综合征模型母鼠胎盘超声造影测量血流灌注与病理微血管密度对比分析

Comparative study on quantitative parameters of blood perfusion and microvessel density in placenta of rat model of preeclampsia evaluted with contrast-enhanced ultrasound

目的探讨妊娠高血压综合征模型母鼠胎盘超声造影（CPS）测量血流灌注参数与肿瘤病理微血管密度（MVD）的相关性。方法制作34只妊娠高血压综合征母鼠动物模型并行超声造影，造影后应用SonoLiver1．025分析软件绘制胎盘的造影时间一强度曲线（TIC），得出妊娠高血压综合征模型母鼠造影定量血流灌注参数达峰时间（TTP）、峰值强度（Imax）、曲线下面积（AUC）及总灌注量（V）；以上所得参数与造影后病理组织免疫组化染色计测的MVD进行相关性分析。结果34只妊娠高血压综合征母鼠胎盘组织TTP为（14．55±3．45）S，Imax为（20．83±6．15）dB，AUC为（1868．61±25．76）dB，V为（58．01±23．56）dB；造影后病理MVD为88．98±24．78；妊娠高血压综合征模型母鼠胎盘Imax与MVD呈正相关（r=0．885，P=0．000），AUC与MVD呈正相关（r=0．677，P=0．001），V与MVD呈正相关（r=0．877，P=0．000），TTP与MVD无相关关系。结论妊娠高血压综合征模型母鼠超声造影Imax、AUC、V与胎盘MVD相关良好，是测量评价妊娠高血压综合征母鼠胎盘血流灌注的可靠依据。

Objective To investigate the correlation between quantitative parameters with contrastenhanced ultrasound （CEUS） and microvessel density （MVD）. Methods Thirty-four placenta of rat model of preeclampsia underwent CEUS examination. The peak intensity time curves on the enhanced images were analyzed quantitatively with computer to get quantitative parameters[the time to peak（TTP）, maximal peak intensity（Imax） ,the area under curve（AUC） and the mean perfusion volume（V）]. These parameters were compared with MVD counted with immunohistochemistry and the correlation was statistically studied. Results The TTP in the enhanced images was （14.55 ± 3.45） s, Imax was （20.83 ± 6.15） dB, AUC was （1868.61 ± 25.76）dB, V was （58.01 ± 23.56）dB, and the MVD of placenta of rat model of preeclampsia was （88.98 ± 24.78） in 34 rats. The Imax was correlated positively to MVD （ r = 0. 885, P = 0. 000） ,AUC was correlated positively to MVD （ r = 0. 677, P = 0. 00l）, V was correlated positively to MVD （ r = 0. 877, P = 0. 000）. There was no correlation between TTP and MVD in lesions. Conclusions The Imax, AUC and V calculated with CEUS were correlated to MVD, these parameters were valuable index for quantitative evaluation of placental blood perfusion.