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煤焦沥青烟提取物致BEAS－2B恶性转化细胞端粒蛋白的变化 被引量：2

The change of telom ere protein in BEAS-2B malignant transformation cell induced by coal tar pitch smoke extracts
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摘要 目的通过检测端粒保卫蛋白复合体中端粒保卫蛋白1（POT1）、端粒重复序列结合因子1（TRF1）和TRF2在煤焦沥青烟致永生化人支气管上皮细胞（BEAS－2B）癌变细胞中的表达变化，探讨其在煤焦沥青烟致肺癌发生中的作用。方法用中温煤焦沥青烟提取物作为诱导剂，建立BEAS－2B的恶性转化模型，用实时荧光定量反转录-聚合酶链反应法（RT—PCR）检测POTI、TRF1和TRF2基因mRNA表达水平；细胞爬片免疫组化半定量法检测其蛋白表达改变。结果BEAS－2B恶性转化细胞POT1和TRF1基因mRNA表达水平（分别为：0．63±0．04，0．36±0．01）和蛋白表达水平（分别为：0．36±0．05，0．09±0．03）均下调，与正常对照组（mRNA：POT1：1．00±0．04，TRF1：1．01±0．16；蛋白：POT1：0．55±0．07，TRF1：0．27±0．07）和二甲亚砜溶剂对照组（mRNA：POT1：0．89±0．12，TRF1：0．90±0．08；蛋白：POT1：0．55±0．10，TRF1：0．26±0．04）相比，差异均有统计学意义（P〈0．05）。BEAS-2B恶性转化细胞TRF2基因mRNA表达水平（1．45±0．07）和蛋白表达水平（0．88±0．06）均上调，与正常对照组（mRNA：1．00±0．07，蛋白：0．48±0．06）和二甲亚砜溶剂对照组（mRNA：1．00±0．06，蛋白：0．50±0．06）相比，差异均有统计学意义（P〈0．05）。结论在煤焦沥青烟致支气管上皮细胞恶性演化过程中，POT1、TRF1和TRF2基因和蛋白表达水平发生变化。 Objective By testing the changes of telomere binding protein in malignant transformation BEAS-2B cells induced by coal tar pitch smoke extracts, to study the role of protection of telomeres 1 （POT1）, telomeric repeat binding factor 1 （TRF1） and TRF2 in tumorgenesis that contact with coal tar pitch. Methods The BEAS-2B cells were induced by coal tar pitch smoke extracts to form malignant transformation cell model in vitro. The gene expression levels of mRNA were assessed by real-time quantitative RT-PCR, the protein expression variations were determined by cell euhure overslip of immunohistoehemical methods. Results In malignant transformation cells, the mRNA expression level （POTI： 0.63±0.04, TRFI： 0.36±0.01） and the protein expression level （POTI： 0.36±0.05, TRFI： 0.09±_0.03） of POT1 and TRF1 was statistically significant decreased compared to that of BEAS-2B group （mRNA： POTI： 1.00±0.04, TRFI： 1.01±0.16; protein： POTI： 0.55± 0.07, TRFI： 0.27±0.07） and DMSO group （mRNA： POTI： 0.89±0.12, TRFI： 0.90±0.08; protein： POTI： 0.55± 0.10, TRFI： 0.26 ±0.04） （P〈0.05）; mRNA expression level （1.45 ±0.07） and the protein expression level （0.88±0.06） of TRF2 was increased compared to that of BEAS-2B group （mRNA： 1.00±0.07, protein： 0.48±0.06） and DMSO group （mRNA： 1.00±0.06, protein： 0.50±0.06） （P〈0.05）. Conclusion The change of gene and protein expression level in POT1, TRF1, and TRF2 involved in the process that evolved into malignant transformation in bronchial epithelial cells BEAS-2B induced by coal tar pitch smoke extracts.
作者 王威 李智涛 祝寒松 赵勇 王丽霞 燕贞 李时恩 许东 吴卫东 吴拥军 吴逸明
机构地区 郑州大学公共卫生学院劳动卫生与职业病学教研室 河南科技大学医学院
出处 《中华劳动卫生职业病杂志》 CAS CSCD 北大核心 2011年第9期678-681,共4页 Chinese Journal of Industrial Hygiene and Occupational Diseases
基金 国家自然科学基金资助项目（30872095,81001239,81001240）
关键词 煤焦油 细胞转化 肿瘤 端粒 重复序列 核酸 Coal tar Cell transformation, neoplastic Telomere Repetitive sequences, nucleic acid
分类号 R73 [医药卫生—肿瘤]
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参考文献18

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共引文献25

同被引文献17

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中华劳动卫生职业病杂志
2011年 第9期

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