鼻咽癌组织中EphA2的表达变化及其意义

The expression and its potentially clinical significance of EphA2 in nasopharyngeal carcinoma

目的:探讨鼻咽癌(NPC)组织中EphA2的表达情况及其与肿瘤发生、发展、侵袭、转移、新生血管生成和预后的相关性。方法:常规免疫组织化学技术检测61例NPC和20例鼻咽部慢性炎症组织中EphA2的表达情况,并分析EphA2的表达与NPC临床病理特征和微血管密度之间的关系。结果:NPC组织EphA2阳性表达率显著高于鼻咽慢性炎症组织(P<0.01)。EphA2阳性表达与NPC临床分期、颈淋巴结和远处转移、5年生存率等临床病理特征有关(P<0.05)。NPC组织中EphA2阳性表达者的微血管密度值显著高于阴性者(P<0.01)。结论:EphA2可能参与NPC的发生、发展、侵袭及转移,促进NPC的新生血管生成,与预后呈负相关。

Objective： To observe the expression of EphA2,and investigate its correlation with the development,progression,invasion,metastasis,angiogenesis and prognosis in nasopharyngeal carcinoma（NPC）. Method： Immunohistochemical staining was used to determine the expression level of EphA2 protein in 61 cases NPC and 20 cases chronic nasopharyngitis samples.The clinically pathological data and results of follow-up were collected.Microvessel density（MVD） was also measured by immunohistochemical staining method with CD34 in NPC. Result： The positive rate of EphA2 protein staining in NPC was 60.66%（37/61）,while that in nasopharyngitis samples was 10.0%（2/20）.The positive rates of EphA2 protein in NPC were 27.27%（3/11） in stage Ⅰ,56.25%（9/16） in stage Ⅱ,68.19%（15/22） in stage Ⅲ,and 83.33%（10/12） in stage Ⅳ.The positive expressions of EphA2 in T1＋T2 and T3＋T4 with neck lymph node and distant metastasis were 58.33%（7/12） and 88.89%（16/18） respectively,while those in T1＋T2 and T3＋T4 without metastasis were 31.25%（5/16） and 50.00%（6/12） respectively.The cumulative survival of patients in the EphA2 positive group at 5 years was only 0.324（12/37）,while 0.500（12/24） in the EphA2 negative group.The positive expression of EphA2 protein was correlated with the clinical stage,the neck lymph node metastasis and distant metastasis,and prognosis of NPC,respectively（P0.05）.MVD in EphA2 protein positive group（45.32±4.91） was significantly higher than that in EphA2 protein negative group（28.69±3.99,P0.01）. Conclusion： EphA2 may play an important role in the development and progression of NPC.It is closely associated with the invasion,metastasis,angiogenesis and prognosis of NPC.