Genistein对人鼻咽癌细胞系CNE抑制增殖和促进凋亡的作用及机制探讨

Effects and mechanism of genistein on the proliferation and apoptosis in human nasopharyngeal carcinoma cell lines CNE

目的:研究genistein对人鼻咽癌细胞系CNE抑制增殖和促进凋亡的作用,探讨其抗癌作用的机制。方法:应用MTT检测不同浓度genistein在不同时间对鼻咽癌细胞系CNE的生长抑制作用;透视电镜下观察鼻咽癌细胞演变过程、凋亡细胞及凋亡小体,应用流式细胞仪分析细胞周期及凋亡率。结果:Genistein可明显抑制CNE细胞的增殖,并且这种抑制作用呈时间及浓度依赖性,随浓度的增加时间的延长抑制作用逐渐增强,尤以200μmol/L组最明显;Genistein诱导鼻咽癌细胞的早期凋亡率随浓度的增加而增强,200μmol/L时为49.9%;FCM分析发现genistein阻断CNE细胞生长于细胞周期的G2/M期;电镜观察到用药后凋亡细胞典型的形态学特征。结论:Genistein对鼻咽癌细胞系CNE的生长有明显的呈时间及浓度依赖性的抑制作用,其阻断细胞的生长主要在细胞周期的G2/M期,并且具有明显诱导CNE细胞凋亡的作用。

Objective： To investigate the effects and mechanism of genistein on proliferation inhibition and onset of apoptosis in nasopharyngeal carcinoma cell line CNE. Method： Antiproliferative effect of genistein against CNE was tested by MTT method.Apoptotic phenotype of CNE was observed by electron microscope.Cell apoptosis percentage and cell cycle phase distribution of CNE were measured by flow cytometric assay. Result： A time dependent and dose dependent proliferation inhibition was demonstrated in CNE,the high potency has the obvious suppression on tumor growth function;Genistein induces nasopharyngeal cancer cell early apoptosis rate but to strengthen along with the density increase,200 μmol/L group is highest,is 49.9%;and genistein induced a G2/ M cell cycle arrest.The characteristic morphological changes of apoptosis in CNE cells was observed after treated by genistein. Conclusion： The time dependent and dosedependent proliferation inhibitory effect of genistein on nasopharyngeal cancer cells.Genisteins mechanism on suppressing effect is probably associated with inducing the cell apoptosis and interving the cell circle of carcinoma cell.The results provide evidence for the potential usefulness of genistein in the prevention and treatment of human nasopharyngeal carcinoma.