胃癌胰腺被膜微转移的研究

Study on pancreatic capsule micrometastasis of gastric cancer

目的探讨胃癌患者胰腺被膜肿瘤微转移的诊断方法。方法采用RT-PCR法,检测CK20 mRNA和CEAmRNA在胃癌患者胰腺被膜组织中的表达。结果 67例胰腺被膜组织CK20 mRNA和CEAmRNA均阳性(微转移阳性)者22例(32.8%)。胃癌胰腺被膜转移与肿瘤的浸润深度、肿瘤的部位、病理分型、临床分期、淋巴结转移有关(P<0.05),而与患者年龄、性别无关(P>0.05)。结论 RT-PCR法检测CK20和CEA的mRNA是诊断胃癌胰腺被膜微转移灵敏方法。胰腺被膜微转移与胃癌的恶性程度正相关,其检测对胃癌根治术的清扫范围有指导意义。

Objective To investigate the diagnostic methods of pancreatic capsule micrometastasis of gastric cancer.Methods The CK20 and CEA mRNA expressions in the pancreatic capsule of patients with gastric cancer were measured by RT-PCR method.Results Of the 67 patients,22 cases（32.8%） had both CK20 and CEA mRNA positive expression（pancreatic capsule micrometastasis-positive） in the pancreatic capsule.The pancreatic capsule micrometastasis of gastric cancer was related to the depth of infiltration,location of the gastric cancer,histological type,clinical stage and lymph node metastasis（P0.05）,but was irrelevant to the age and sex of the patients（P0.05）.Conclusions The measurement of CK20 and CEA mRNA by RT-PCR is a sensitive method for diagnosis of pancreatic capsule micrometastasis of gastric cancer.The pancreatic capsule micrometastasis is positively correlated with the malignant degree of gastric cancer and it can be a guide for resection range of gastric cancer.