上海地区HIV/HCV重叠感染者HCV准种的分子流行病学研究

Molecular epidemic of HCV quasispecies variability in HIV/HCV coinfection patients from Shanghai

目的观察HIV/HCV重叠感染和高效抗反转录病毒治疗(HAART)对HCV准种的影响。方法通过PCR、测序及单链构象多态性分析建立HCV高变1区(HVR1)准种变异率检测方法,运用该方法对我国上海地区48例HIV/HCV重叠感染者HCV准种变异的分子流行病学进行研究。结果与单独HCV感染组和HIV/HCV重叠感染且已行HAART组相比较,HIV/HCV重叠感染但未行HAART组的HCV准种变异率较低,差异有统计学意义(P均<0.05)。单独HCV感染组与HIV/HCV重叠感染且已行HAART组之间作比较,HCV准种的复杂性和多样性差异均无统计学意义。结论 HIV感染可降低HCV准种变异率。随着HAART长期应用,HIV/HCV重叠感染者的HCV准种率增加。

Objective To observe the molecular epidemic effects of human immunodeficiency virus（HIV）/HCV coinfection and HAART on HCV quasispecies variability.Methods We detected quasispecies of HCV hypervariable region 1（HVR1）by PCR,nucleotide sequencing and single-stranded conformation polymorphism method.We used the method in 48 subjects from Shanghai and observed the molecular epidemiology of HCV quasispecies variability in HIV-HCV coinfection of the region.Results This study shows that HIV-positive,HAART-naive subjects had significantly lower HCV quasispecies complexity and diversity than HIV-negative and HIV-positive HAART-treated subjects（P0.05）.Conclusion HIV coinfection is associated with decreased HCV quasispecies variability,which appears to be reversed by effective HAART.