活化嗜碱粒细胞在变应性鼻炎患者外周血中的表达

Expression of activated basophile in peripheral blood in patients with allergic rhinitis

目的探讨嗜碱粒细胞在变应性鼻炎患者外周血中数量及活化率的变化及其临床意义。方法以化学诱导趋向性受体单抗作为特异性的荧光标记,采用三色免疫荧光标记流式细胞术对20例急性发作期变应性鼻炎患者(急性组)、20例反复发作变应性鼻炎患者(慢性组)及30例健康者(对照组)的外周血嗜碱粒细胞及其活化率进行定量检测。结果慢性组外周血化学诱导趋向性受体及嗜碱粒细胞高于急性组(P<0.01)和对照组(P<0.05),对照组高于急性组(P<0.01)。慢性组与急性组嗜碱粒细胞活化率均高于对照组(P<0.01)。结论嗜碱粒细胞活化是导致变应性鼻炎症状出现的主要因素之一。慢性期患者嗜碱粒细胞的持续增高和活化可能与变应性鼻炎反复发作有关。

Objective To evaluate the number and the activation rate of basophile in peripheral blood in patients with allergic rhinitis and their clinical significance.Methods The chemoattractant receptor-homologus molecule expressed on T helper cells was used as a specific fluorescent marker.Twenty patients with acute allergic rhinitis,20 patients with chronic allergic rhinitis and 30 healthy controls were evaluated the number and the activation rate of basophile in peripheral blood with three-color fluorescent flow cytometry analysis.Results The numbers of chemoattractant receptor-homologus molecule expressed on T helper cells and basophile were significantly larger in chronic allergic rhinitis group than those in acute allergic rhinitis group（P0.01） and normal group（P0.05）.The activation rate of basophile was significantly higher in acute allergic rhinitis and chronic allergic rhinitis group than that in control group（P0.01）.Conclusion The activation of basophile is one of the main factors inducing the symptoms of allergic rhinitis.The continuous increase of the number and the activation rate of basophile may be involved in the recurrence of chronic allergic rhinitis.