大豆异黄酮在低雌激素环境下对MMTV-erbB-2转基因小鼠的作用

Effect of soybean isoflavones on MMTV-erbB-2 transgenic mice in the low estrogen environment

目的探讨大豆异黄酮在低雌激素环境下对MMTV-erbB-2转基因小鼠乳腺肿瘤发生、发展的影响。方法 MMTV-erbB-2转基因雌性小鼠90只,分为3组,每组30只。60只行卵巢摘除术使MMTV-erbB-2转基因小鼠体内形成低雌激素环境,分别使用含大豆异黄酮饲料(大豆异黄酮组)和普通饲料(普通饲料组)喂养。另30只为对照组,用普通饲料喂养。观察乳腺肿瘤的潜伏期及发生情况,用免疫组织化学法观察各组乳腺组织的雌激素受体、孕激素受体、增殖细胞核抗原的表达情况。结果大豆异黄酮组肿瘤潜伏期较其他2组明显缩短,增殖细胞核抗原表达水平较其他2组高(P<0.05)。3组雌激素受体、孕激素受体表达情况比较差异无统计学意义(P>0.05)。结论在低雌激素环境下,大豆异黄酮对MMTV-erbB-2转基因小鼠乳腺肿瘤的发生、发展起促进作用。

Objective To study the influence of soybean isoflavones on occurrence and development of breast cancer in MMTV-erbB-2 transgenic mice.Methods In 90 female MMTV-erbB-2 transgenic mice,60 mice received surgical castration to create a low estrogen environment,in which 30 mice were fed with soy isoflavones diet（soy isoflavones group） and 30 mice were given normal diet.The other 30 mice receiving normal diet were as controls（control group）.The latency and occurrence of breast tumor were observed.Immunohistochemical staining was used to detect the expressions of estrogen receptor,progesterone receptor and proliferating cell nuclear antigen.Results The tumor latency was obviously shorter and the expression rate of proliferating cell nuclear antigen was significantly higher in soy isoflavones group than that in the other two groups（P0.05）.There were no significant differences in the expressions of estrogen receptor and progesterone receptor in these three groups（P0.05）.Conclusion Soy isoflavones in low estrogen environment plays a catalytic role in the occurrence and development of breast tumor in MMTV-erbB-2 transgenic mice.