鲍曼不动杆菌外膜蛋白研究进展

Study on outer membrane proteins of Aeinetobacter baumannii

鲍曼不动杆菌是医院获得性感染主要致病菌之一，其外膜蛋白是指肽聚糖外脂质双层中间镶嵌的一些特殊的蛋白质。CarO、33-to-36-kDa、OprD等外膜蛋白丢失与细菌耐药有关，而OmpA外膜蛋白是重要的致病因素。其研究技术分为提取、分离和鉴定3个方面，超声物理法、双向凝胶电泳、质谱技术及免疫印迹是目前常用技术。

Acinetobaeter baumannii is one of the major pathogens in nosocomial infection, its outer membrane proteins are some special proteins embedded in lipid bilayer outside the peptidoglycan. The loss of CarO,33-to36-kDa, OprD and other outer membrane proteins is related with drug resistance, and outer membrane protein A is an important pathogenic factor. Its research technology including three aspects of the extraction, separation and identification, the ultrasound physical method, 2-Dimensional Gel Electrophoresis, mass spectrometry and western blot analysis are commonly used now.