植物甾醇烟酸酯的合成及HPLC法的测定

Synthesis of bionic ester of phytosterol and analysis of it by HPLC

采用两步法合成植物甾醇烟酸酯,产物总收率为87.4%。通过液质联用仪对产物进行验证。豆甾醇烟酸酯为标准物,应用高效液相色谱法对产物中植物甾醇烟酸酯的含量进行了分析测定。以Eclipse XDB-C18柱(4.6mm×150 mm,5μm)为分析柱,甲醇为流动相,流速是1.2 mL/min,紫外210 nm检测,柱温35℃。实验结果表明,该方法快速、简便、分离度好。

The overall yield of the two-step synthesising bionic ester of phytosterol was 87.4% and the production was validated by liquid chromatograph/mass spectrometer（HPLC/MS）.Homemadebionic ester of stigmasterol for the standard substance,The HPLC methods for bionic ester of phytosterol was established,Chromatographic condition：reversed-phase column（150 mm×4.6 mm）filled with 5 μm Eclipse XDB C18 Column as the solid phase,mobile phase was CH3OH,flow-rate was 1.2 mL/min,detection wavelength was 210 nm,column temperature was 35℃.The experimental results showed that the method was simple,sensitive and accurate.