繁殖期乌鳢、鲫鱼腺垂体内分泌细胞的体视学观察

Stereology observations of endocrine cells in adenohypophysis during reproduction period of mullet and crucian

为探讨繁殖期乌鳢、鲫鱼腺垂体的组织学结构,选用H.E.染色法、Azan三色染色法、PbH染色法、PAS-OG染色法及Jafri鱼类脑垂体复合染色法对其腺垂体进行光镜组织学观察并应用体视学方法对它们腺垂体中内分泌细胞的百分数、体积密度、平均体积及数密度进行了测算。结果表明:①乌鳢的脑垂体为前后型,鲫鱼的脑垂体为背腹型。②经不同种染色法可以显示出腺垂体中的不同种内分泌细胞。③经不同种脑垂体染色方法染色后同种鱼腺垂体中同种内分泌细胞的百分数、体积密度、平均体积及数密度均无显著性差异(P>0.05)。④经同种染色方法染色后两种鱼腺垂体中的6种内分泌细胞的百分数、体积密度、平均体积及数密度均有显著性差异(P<0.05)。

Using five different staining methods（i.e.,H.E.staining method,Azan（Mallory-Heidenhain-Azan） staining method,PbH（plumbum hematoxylin） staining method,PAS-OG（periodic acid Schiff＇s orange G） staining method and Jafri combined staining method for fish pituitary）,the histological structure of adenohypophysis during reproduction period in mullet and crucian was observed in depth.Stereology method was applied to measure and calculate the percentage,volume density,average volume and numerical density of the endocrine cells in these two kinds of fish.The results indicated that：1） The pituitary was of front-back style in mullet and dorsoventral style in crucian.2） Different kinds of endocrine cells could be distinguished in adenohypophysis,with different and special pituitary staining methods.3） No distinct difference was found between the endocrine cells in adenohypophysis in percentage,volume density,average volume and numerical density using different pituitary staining method in one kind of fish（P0.05）.4） It existed distinct difference in percentage,volume density,average volume and numerical density of the endocrine cells in adenohypophysis between the two kinds of fish using the same pituitary staining method（P0.05）.