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小鼠B7-H3增强体外脂多糖介导的单核细胞活化 被引量：3

Murine B7-H3 Enhancing to the Lipopolysaccharide Activated Monocytes
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摘要 目的利用已成功构建的小鼠B7-H3融合蛋白通过体外实验研究B7-H3对单核细胞活化及功能的调节作用。方法采用磁珠法从小鼠脾脏中分离得到CD11b+单核细胞,经脂多糖活化后通过融合蛋白结合实验检测单核细胞表面B7-H3受体的表达;加入不同剂量小鼠B7-H3融合蛋白培养,ELISA法检测单核细胞IL-1β、TNF-α与IL-6的分泌,并通过吞噬实验分析其对单核细胞吞噬功能的影响。结果活化的小鼠单核细胞表面可能存在B7-H3的未知受体;与对照相比,小鼠B7-H3可以增加活化的单核细胞IL-1β、TNF-α与IL-6的分泌(均P<0.01),并增强单核细胞的吞噬能力。结论小鼠B7-H3分子在感染免疫中具有重要作用,可参与疾病的抗感染功能的发挥。 Objective To discuss the B7-H3 regulation and function to monocytes using murine B7-H3 fusion protein.Methods CD11b＋ cells were separated from splenocyte suspensions using a mouse CD11b positive selection kit,then the expression of B7-H3 receptor on monocytes was analyzed after the cells was activated by lipopolysaccharide for 24 hours.Cells were incubated with B7-H3-Ig-fusion protein with LPS for 24h and the IL-1β,TNF-α and IL-6 were detected through ELISA.At the same time the changes were measured in phagocytic activity of monocytes with FITC-dextran.Results There might be a potential receptor for B7-H3 on mouse monocyte.Murine B7-H3 could increase the secreting of IL-1β,TNF-α and IL-6 relative to the controls.Additionally the gene could enhance the phagocytosis of monocyte.Conclusion Murine B7-H3 plays an important role in infective immunity to take part in the disease counteracting.
作者 孙静 严茹红 顾文超 张光波 傅丰庆 张学光
机构地区 苏州卫生职业技术学院 苏州大学医学生物技术研究所 苏州大学附属第二医院
出处 《苏州大学学报（医学版）》 CAS 北大核心 2011年第4期535-537,585,共4页 Suzhou University Journal of Medical Science
基金 国家自然科学基金资助项目(31040022)
关键词 小鼠B7-H3 单核细胞 感染免疫 脂多糖 murine B7-H3 monocyte infective immunity lipopolysaccharide
分类号 R392.12 [医药卫生—免疫学]
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  • 1Lenschow DJ,Walunas TL,Bluestone JA,et al.CD28/B7 system of T cell costimulation[J].Annu Rev Immunol,1996,14:233-258.
  • 2Sharpe AH,Freeman GJ.The BT-CD28 superfamily[J].Nat Rev Immunol,2002,2(2):116-126.
  • 3Chapoval AI,Ni J,Lau JS,et al.B7-H3:a costimulatory molecule for T cell activation and IFN-γ production[J].Nat Immunol,2001,2(3):269-274.
  • 4SUn M,Richards S,Prasad DV,et al.Characterization of mouse and human B7-H3 genes[J].J Immunol,2002,168(12):6294-6297.
  • 5Wang L,Fraser CC,Kikly K,et al.B7-H3 promotes acute and chronic allograft rejection[J].EurJ Immunol,2005,35(2):428-438.
  • 6Iuo L,Chapoval AI,Flice DB,et al.B7-H3 enhances tumor immaunity in by costimulating rapid clonal expansion of antigen-specific CD8+ cytolytic T cells[J].J Inmmunol,2004,173(4):5445-5450.
  • 7Prasad DV,Zhang RN,Li ZX,et al.Murine B7-H3 is a negative regulator of T cells[J].J Immunol,2004,173(4):2500-2506.
  • 8Ling V,Wu PW,Spaulding V,et al.Duplication of primate and rodent B7-H3 immunoglobulin V-and C-like domains:divergent history of functional redundancy and exon loss[J].Genomics,2003,82(3):365-377.
  • 9Sub WK,Gajewska BU,Okada H,et al.The B7 family member B7-H3 preferentially down-regnlates T helper type 1-mediated immune respenses[J].Nat Immunol,2003,4(9):899-906.

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  • 1薛辛东.杜立中,毛萌.儿科学[M].2版.北京:人民卫生出版社,2010:263-290.
  • 2Ramakrishnan M, Ulland A J, Steinhardt LC, et al. Sequelae due to bacte- rial meningitis among African children: A systematic literature review [ J ]. BMC Med ,2009,7:47.
  • 3Luan Y,Ju J ,Luo L,et al. Potential role of soluble B7 - H3 in liver im- munopathogenesis during chronic HBV infection [ J ]. J Viral Hepat, 2012,19(1) :23 -31.
  • 4uan HX, Wei XD, Zhang GB, et al. B7 - H3 over expression in prostate cancer promotes tumor cell progression [ J ]. J Urol, 2011,186 ( 3 ) : 1093 - 1099.
  • 5O'Brien KL, Wolf son LJ, Watt JP, et al. Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years : Global esti- mates [ J . J Lancet,2009,374 (9693) :893 - 902.
  • 6Barichello T, dos Santos I, Savi GD, et al. TNF - ct, IL - 113, IL - 6, and cine - 1 levels in rat brain after meningitis induced by Streptococcus pneumoniae[ J]. J Neuroimmuno1,2010,221 ( 1 -2) :42 -45.
  • 7Li H, Nourbakhsh B, Cullimore M, et al. IL - 9 is important for T - cell activation and differentiation in autoimmune inflammation of the central nervous[ J . Eur J Immuno1,2011,41 (8) :2197 - 2206.
  • 8Yi KH, Chen L. Fine tuning the immune response through B7 - H3 and B7 - H4 [ J ]. lmmunol Rey,2009,229 ( 1 ) : 145 - 151.
  • 9Hashiguchi M, Kobori H, Ritprajak P, et al. Triggering receptor ex- pressed on myeloid cell - like transcript 2 ( TLT - 2 ) is a counter - re- ceptor for B7 - H3 and enhances T cell responses [ J ]. Proc Natl Acad Sc U S A ,2008,105(30) :10495 - 10500.
  • 10Chen X,Zhang G,Li Y,et al. Circulating B7 -H3(CD276) elevations in cerebrospinal fluid and plasma of children with bacteria[ meningitis [J]. J Mol Neurosci,2009,37( 1 ) :86 -94.

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苏州大学学报（医学版）
2011年 第4期

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