原儿茶醛在孕羊体内的药代动力学研究

Study on Pharmacokinetics of Protocatechuic Aldehyde in Pregnant Sheep

目的研究丹参冻干粉中原儿茶醛在孕羊体内的药代动力学过程。方法在清醒无应激条件下,母羊静脉给予不同剂量(5、10、20 mg/kg)丹参冻干粉,采用高效液相色谱内标法测定母羊静脉给予丹参冻干粉后不同时间(5、30、60、90、120、240 min)血浆中原儿茶醛的浓度,用Bapp6.0药动学软件计算其药代动力学参数。结果母羊静脉给予不同剂量丹参冻干粉后5、30、60、90 min均可测得母羊血浆中原儿茶醛浓度,且与丹参给药剂量成一定的线性关系。原儿茶醛的血浆半衰期为60 min左右,它在母羊体内吸收很快,达峰时间为5 min,而后以较快速度消除。结论研究丹参在母羊体内的药代动力学过程可为临床应用丹参防治妊娠期疾患时制定合理的给药方案提供依据。

Objective To determine pharmacokinetics of protocatechuic aldehyde in Danshen freeze-dry injection in the conscious and unstressed pregnant sheep in vivo.Method Three doses of Denshen were intravenously administrated into the femoral vein via the catheter.After the drug administration,maternal blood samples were collected for high performance liquid chromatography（HPLC） analysis in determination of Danshen concentrations by measuring protocatechuic aldehyde levels in maternal blood.Results At the 5 th,30 th,60 th,and 90 th minute following intravenous administration of different doses of the drug（5,10,and 20 mg/kg） in the maternal sheep,protocatechuic aldehyde was detected in maternal blood.The half-life period of the drug in maternal blood was about 60 mins.There was dose-dependent influence for blood Danshen concentrations.Conclusion The present study demonstrates that the pharmacokinetics of protocatechuic aldehyde in Danshen freeze-dry administration in pregnant sheep,providing new information for reasonable dosage regimen for clinical use of Danshen during pregnancy.