雄性SD大鼠各泌尿生殖器官感染解脲支原体阳性率及病理改变的比较研究

A Comparative Study of the Infection Rates and Pathologic Changes by Ureaplasma Urealyticum in Urogenital Organs in Male SD Rats

目的了解解脲支原体(Uu)对雄性SD大鼠各泌尿生殖器官感染情况,为预防及治疗Uu感染提供理论依据。方法取性成熟雄性SD大鼠40只,晨尿离心,沉渣作Uu液体培养,随机取Uu培养阴性30只随机分为两组,A组为模型组共22只;B组为对照组共8只。A组每只动物经膀胱注入血清8型Uu菌株0.5 ml,浓度为5×106CCU/ml;B组每只动物膀胱注入等量生理盐水。第40天再次取晨尿离心,沉渣作Uu液体培养,阳性者做固体培养,再次培养阳性者,定为造模成功。再分别取造模成功的大鼠各泌尿生殖系统器官组织作组织培养及病理检查。结果模型组19只(死亡3只)大鼠组织培养阳性,各泌尿生殖系统器官Uu阳性感染率比较差异均有统计学意义(均P<0.05),以尿道组织培养阳性率最高(84.2%),其次依序为前列腺与睾丸均为63.2%,附睾、精囊、输精管均为47.4%,膀胱42.5%。前列腺与睾丸病理有明显改变,其余组织无明显改变。对照组8只培养均为阴性。结论 Uu感染可广泛并不同程度浸润雄性大鼠泌尿生殖器官,同时提示Uu最易侵犯的器官为尿道、前列腺及睾丸,其次为附睾、精囊、输精管、膀胱,但组织病理改变仅少数器官有变化,且均无明显炎症细胞浸润。

Objective To investigate infection of the urogenital organs in male SD rats caused by ureaplasma urealyticum so as to provide theoretical basis for research into its prevention and treatment.Methods Forty sexually mature male SD rats whose urina sanguinis was centrifuged were taken in the study,and the sediments were cultured for ureaplasma urealyticum.Thirty randomly selected negative rats were randomly divided into group A of 22 as the model group and group B of 8 as contrast group.Each rat in group A was injected with 0.5 ml（5×106CCU/ml） of serum ureaplasma urealyticum type 8 into bladder and in Group B with the same amount of normal saline.On the 40th day,urina sanguinis was collected and centrifuged,and the sediments were cultured in fluid for ureaplasma urealyticum.Culture on solid media was conducted in the positive ones.The model was established if the cultures on solid media were positive.Tissues of urogenital organs in the model group were obtained from for tissue cultures and pathological examination.Results Tissue cultures of 19 rats in model group（3 deaths） were positive.The differences in infection rates in urogenital organs were statistically significant（P0.05） with the highest of 84.2% in urinary tract,63.2% in both prostate and testicle,47.4% in epididymis,seminal vesicle and seminferous duct respectively,and 42.5% in bladder.Tissue cultures of the 8 rats in the control group were all negative.Evident pathological changes were seen in prostates and testicles but not in others organs.Conclusion Ureaplasma urealyticum can extensively affect urogenital organs in the male rats and severity of infection in different organs varies.The results also suggest that the most susceptible are urinary tract,prostate and testicle,followed by pididymis,seminal vesicle,seminiferous duct,and bladder.However,pathological changes occur in a limited number of organs,and no obvious inflammatory cell infiltration is observed in all organs surveyed.