山西省黄瓜灰霉病菌对乙霉威的抗药性检测

The Resistance Detection to Diethofencarb in Botrytis cinerea Pers.in Shanxi Province

为了明确山西主要蔬菜种植区黄瓜灰霉病菌对乙霉威的敏感性,采用组织分离法从罹病黄瓜上得到176株灰霉病菌菌株(Botrytis cinerea Pers.),并采用最低抑制浓度法(MIC)和菌落直径生长法分别测定了176株菌株的抗性频率和抗性水平。结果表明:来自8个市区的176株黄瓜灰霉病菌菌株对乙霉威的抗性频率为77.84%,平均抗性水平为312.1倍,其中,敏感菌株39株,低抗菌株46株,中抗菌株28株,高抗菌株50株,特高抗菌株13株。表明山西黄瓜灰霉病菌对乙霉威的抗性程度增强,建议在生产中减少乙霉威的使用或与其他药剂混合使用。

To study the resistant level of Botrytis cinerea Pers.to diethofencarb,176 Botrytis cinerea strains were isolated adopting tissue segregation method from 8 vegetable cultivation bases in Shanxi province.The resistance frequency and the resistance level were detected by the methods of minimum inhibitory concentration（MIC）and growth of colonies diameter.The results showed that the resistance frequency of the strains was 77.84%,and the average resistance level was 312.1 times.Among the strains,there were 39 sensitive resistant strains,46 low resistant strains,28 moderate resistant strains,50 high resistant strains and 13 super resistant strains.It indicated that the resistance level of Botrytis cinerea Pers.to diethofencarb in Shanxi province increased.It was recommend to reduce use of diethofencarb,or in its using mix it with other fungicides.