可手术胃癌血清HER-2/neu ECD水平与临床特征及疗效的研究

Research between the serum levels of HER-2/neu ECD in operable gastric cancer patients and clinical characteristics and efficacy

目的:探讨可手术胃癌患者血清HER-2/neu胞外域部分(ECD)水平与临床特征及疗效的关系。方法:收集江苏省连云港市第二人民医院2010年01月至2010年12月,可手术胃癌患者46例进入研究,术前、术后收集血清,同时获取15例正常人、22例胃良性病变患者血清为对照组。另外收集7例血清HER-2/neuECD阳性患者四周期化疗后血清。采用酶联免疫吸附方法(ELISA)检测血清HER-2/neu ECD水平。结果:胃癌患者中,7/46(15%)血清HER-2/neu ECD水平高于正常;血清HER-2/neu ECD水平与患者年龄,性别,T分期,淋巴结转移,术后病理分期及肿瘤分化程度均无相关性(P>0.05);胃癌术后血清HER-2/neu ECD水平较术前有明显的降低,四周期辅助化疗后较术后血清HER-2/neu ECD水平无明显降低。结论:可手术胃癌患者血清HER-2/neu ECD水平与年龄,性别,T分期,淋巴结转移,术后病理分期及肿瘤分化程度均无相关性;血清HER-2/neu ECD水平可以作为疗效判断的指标。

Objective. To study the relationship between the serum levels of HER-2/neu extracel- lular domain （ECD） and clinical Characteristics and efficacy in operable gastric cancer patients. Methods： We Collected serum samples of 46 cases of gastric cancer patients（before and after operation）, 15 cases of healthy people,22 cases of gastric benign lesion and 7 case of the positive levels of HER-2/neu ECD in serum after four cycles of adjuvant chemotherapy;using enzyme linked immunosorbent assay （ELISA） to test the HER 2/neu ECD levels in serum samples of all patients. Results： Among the patients with gastric cancer, 7/46 （15） of them had a higher levels of HER-2/neu ECD than normal ones. The levels of HER-2/neu in serum were not associated with age, gender, T stage, lymph node metastasis, postopera- tive pathologic stage and tumor differentiation degree（P：~0.05）. The levels of serum HER-2/neu ECD af- ter operation were reduced much more than the ones before operation significantly, the levels of serum HER-E/neu ECD were not going to decrease significantly after four cycles of adjuvant chemotherapy. Conclusion： The levels of HER-2/neu ECD in serum were not associated with age, gender, T stage, lymph node metastasis, postoperative pathologic stage and tumor differentiation degree; The levels of Her-2/neu ECD in serum can be used as index to determine efficacy.