表皮生长因子受体在上皮性卵巢癌组织中的表达及其与预后的关系

Expression of EGFR in epithelial ovarian cancer and their correlation with prognosis of patients

目的:检测上皮性卵巢癌组织中表皮生长因子受体(EGFR)mRNA及蛋白的表达水平,分析其与卵巢癌临床病理特征及预后的关系。方法:采用RT-PCR和免疫组化的方法检测EGFR在上皮性卵巢癌、卵巢良性肿瘤和正常卵巢组织中的表达。结果:在卵巢癌组织中,EGFR mRNA的阳性表达率为64.44%(29/45),相对表达水平为0.81±0.23,均明显高于卵巢良性肿瘤和正常卵巢组织,差异均具有统计学意义(P<0.05～0.01)。EGFR在有淋巴结转移的癌组织中的相对表达量明显高于无淋巴结转移组,FIGO分期Ⅲ～Ⅳ期癌组织中表达高于Ⅰ～Ⅱ组,低分化癌组织的表达量明显高于中、高分化癌组织,差异均有统计学意义(P<0.05～0.01)。在卵巢癌组织中,Long-rank分析显示EGFR阳性表达患者的累积生存率明显低于阴性者,差异有统计学意义(P<0.01)。结论:EGFR在上皮性卵巢癌组织中高表达,并且与卵巢癌的组织分级、临床分期、淋巴结转移及患者的生存期密切相关,可作为判断上皮性卵巢癌恶性生物学行为和预后的重要参考指标。

Objective：To investigate the expression of Epidermal Growth Factor Receptor（EGFR） in epithelial ovarian cancer and their correlation with clinical pathological tients. Methods： Expression of EGFR mRNA in ovarian cancers, benign features and prognosis of the paovarian tumor and normal ovarian tissues were detected by RT-PCR assay and immunohistochemical staining methods. Results. The positive rate of EGFR expression in tumor tissues was 64.44 %, with an average expression level of （0.81＋0.23）, both data were significant higher than that in benign tumors and normal tissues （P〈0.05）. The EGFR expression level were significant higher in cancerous tissues with lymphatic metastasis than that without lymphatic metastasis, in stage Ⅲ～Ⅳ canner than that in stage Ⅰ～Ⅱ cancer, in （P〈0. 05-0.01）. Long-rank analyze indicated the accumulated survival rate of the initially differentiate cancer tissues than that in mediate and highly differentiate tissues （P〈0.05-0.01）. For patients with cancer, long-rank analysis showed that patients showed positive EGFR expression had lower cumulative survival rate than those showed negative results. Oonclusions： The high expression level of EGFR in epithelial ovarian cancer is closely correlated with stage of epithelial ovarian cancer stage, lymphatic metastasis status and patientsr survival rate, thus it can be used as a significant indicator of the biological behavior and patientsr prognosis.