二甲苯对小鼠肺组织损伤的初步研究

Preliminary Study on the Damage in Lungs of Mice Exposed to Xylene

为了研究二甲苯对小鼠肺组织的损伤情况,以健康成年小鼠作为试验动物,分3个试验组(低、中、高剂量组)1个对照组,给试验组腹腔注射不同剂量二甲苯(0.125、0.25、0.5 mL/kg),给对照组腹腔注射生理盐水。15 d后剖检取材,采用石蜡切片和HE染色技术研究二甲苯染毒后小鼠肺组织结构的变化特点;采用分光光度法测定小鼠肺组织超氧化物歧化酶(SOD)的活性和丙二醛(MDA)的含量。结果表明,二甲苯导致小鼠肺组织结构发生不同程度的病理变化,肺泡间隔增厚且有出血现象、周围有炎症细胞侵润。与对照组相比,各试验组肺组织内SOD活力随二甲苯剂量的增加而降低;MDA含量随二甲苯剂量的增加而升高,且各试验组之间SOD和MDA的含量差异显著。本试验结果提示,二甲苯对小鼠肺组织具有损伤作用,且随二甲苯剂量的增加肺组织损伤程度越来越严重。

In order to study the damage of xylene in the lung tissue of mice,the healthy adult mice were divided in 3（high,middle,low dose） experimental groups and 1 control group at random.The mice in exposed groups were injected with different doses of xylene（0.5,0.25,0.125 mL/kg）,and the mice in control group were injected with equal physiological saline solution by intraperitoneal injection once a day.Histological structures of lungs in mice esposed to different doses of xylene were examined by HE stanning of paraffin section;the activity of the superoxide dismutase（SOD） and the content malondialdehyde（MDA） of lungs in mice exposed to xylene were measured by spectrophotometric method.The result showed that the xylene may lead to pathological lesions in lungs.Compared with the control,the alveolar septa were thicker in mice exposed to xylene,there was more hemorrhage and inflammatory cell infiltration in the alveolar septa.The activities of SOD of lungs in mice were significantly decreased,while the levels of MDA were significantly increased in the high-and middle-dose groups compared with mice in control group.It is suggested that xylene may cause damage in the lungs of mice and the damage is more serious with the increasing dose of xylene.