哈萨克、汉族食管癌p16基因甲基化及其表达的研究

Study on methylation and expression of p16 in esophageal squamous cell carcinoma in the Hazak and Han nationality

目的探讨新疆哈萨克族、汉族食管癌患者p16基因的甲基化状态及其与食管癌发生的关系。方法采用甲基化特异性聚合酶链反应(MSP)的方法,检测哈萨克族(36例)、汉族(35例)食管癌患者及正常人群对照组p16基因的甲基化状态,分析其与年龄、性别、淋巴结转移、分化程度及临床病理分期等的关系。结果 36例哈萨克族食管癌癌组织、癌旁组织的甲基化率分别为44.4%、13.9%,正常人群对照组的甲基化率为2.8%;35例汉族食管癌癌组织、癌旁组织的甲基化率分别为42.9%、14.3%,正常人群对照组的甲基化率为2.9%。食管癌癌组织p16基因的甲基化率显著高于癌旁组织和正常人群对照组,差异有统计学意义(P<0.05),p16基因启动子区甲基化与肿瘤分化程度密切相关。结论新疆哈萨克族、汉族食管癌患者中p16基因启动子甲基化与食管癌的发生有密切的关系。

Objective To explore the relationship between the level of methylation of the p16 gene and the occurrence of esophageal squamous cell carcinoma in the Hazak and Han.Methods The methylation of the p16 gene was detected by methylation specific polymerase chain reaction（MSP） in 71 esophageal cancer tissues and adjacent tissues.Results The methylation rates of p16 gene in esophageal cancer tissues and adjacent tissues were 44.4%,13.9% in Hazaks′,and 42.9%,14.3% in 35 Hans′.The methylation rates of p16 gene in normal group was 2.8% in 36 Kazaks,and 2.9% in 35 Hans.Significant differences were seen in the methylation rates of p16 gene in esophageal cancer tissues and adjacent tissues and normal group（P0.05）.Conclusion Aberrant promoter methylation of p16 promoter region maybe an early event and play an important role in the pathogenesis of esophageal squamous cell carcinoma in the Hazak and Han nationality.