新疆柯尔克孜族人群血小板抗原1～6、15系统基因多态性分析

Analysis on the polymorphism of Human Platelet Antigen (HPA) 1～6 and 15 genes systems in Xinjiang Khalkhas

目的探讨新疆柯尔克孜族人群人类血小板抗原HPA1-6,15(Gov)系统多态性,分析HPA基因在柯尔克孜族人群中的遗传多态性。方法采用聚合酶链-序列特异性引物(PCR-SSP)法105例无血缘关系的新疆柯尔克孜族血样进行HPA基因分型。结果新疆柯尔克孜族HPA-1a、2a、3a、4a、5a、6a、15a基因频率分别是0.900 0、0.923 80、.576 2、1、0.952 4、10、.566 7;HPA-1b、2b、3b、4b、5b、6b、15b基因频率分别是0.100 00、.076 2、0.423 8、0、0.047 6、00、.433 3,新疆柯尔克孜族HPA基因频率与新疆汉族相比,HPA-1a、1b、2a、2b、15a、15b基因频率差异具有统计学意义(P<0.05)。与新疆维吾尔族相比,HPA-15a、15b基因频率差异具有统计学意义(P<0.05)。结论新疆柯尔克孜族人群中HPA-1、2、3、5、15系统均具有多态性,其中HPA-3,15系统具有高度多态性,这使免疫暴露的机会增加。

Objective To investigate the polymorphism of Human Platelet Antigen（HPA）1～6,15 genes systems in Xinjiang Khalkhas.MethodsHPA genotyping among 105 unrelated volunteer donorswas conducted via PCR-SSP.ResultsGene frequencies for HPA-1a,2a,3a,4a,5a,6a and 15a were 0.900 0,0.923 8,0.576 2,1,0.952 4,1,and 0.566 7,respectively.Gene frequencies for HPA-1b,2b,3b,4b,5b,6a,15b were 0.100 0,0.076 2,0.423 8,0,0.047 6,0 and 0.433 3,respectively.Compared with xinjiang Han nationality,the HPA-1,2,15 systems gene frequencies of Khalkhas showed significant difference（P0.05）.Compared with Uygurs,the HPA-15 system gene frequencies of Khalkhas showed significant difference（P0.05）.ConclusionThe results suggest high polymorphism in HPA-1,-2,-3,-5 and-15 systems among Xinjiang Khalkhas.Among them,HPA-3 and HPA-15 showed a high polymorphism,which increase the incidence for immunological exposure.