广西眼镜蛇遗传多样性RAPD分析

Genetic diversity analysis of Guangxi Naja naja atra by RAPD analysis

对广西眼镜蛇的血液基因组进行RAPD扩增,从30条随机引物中筛选出18条可扩增出清晰多态条带的引物。结果表明,18条随机引物共产生了257个位点,其中多态性位点152个,多态率为59.1%;个体间的平均遗传相似系数为0.8186;个体间平均遗传距离为0.1814,群体Shannon信息指数为0.3170;Nei’s基因多样性指数为0.2112;等位基因观察数为1.3563;有效等位基因数为1.5914。表明广西眼镜蛇具有较强的遗传多样性。

PCR amplification was conducted for genome DNA pool of Guangxi Naja naja atra using 30 random primers. 18 random primers were selected to amplify genome DNA of Guangxi Naja naja atra. The results showed that 257 loci were detected, 152 of which （59.14%） were polymorphie. The genetic similarity index and average genetic distance were 0.8186 and 0.1814, respectively.The Shannon index and Nei＇s gene diversity were 0.3170 and 0.2112, respectively. The alleles observed number and effective numbers of alleles were 1.3563 and 1.5914, respectively. The research results showed that there was relatively higher genetic diversity in Guangxi Naja naja atra.