基于双单倍体牙鲆挖掘与生长性状连锁的微卫星标记

Development of microsatellite markers correlation to growth traits based on double haploids of Japanese flounder

利用中国水产科学研究院北戴河中心实验站2007年制备的双单倍体牙鲆为材料,根据生长性状表型值分成快速生长组（Fast growth group,FGG）和慢速生长组（Slow growth group,SGG）,进行微卫星标记与生长性状的连锁分析。从牙鲆遗传连锁图谱选择40个高多态性的微卫星标记,覆盖了24个连锁群中的22个。结果表明,FGG和SGG每组各30尾个体进行40个微卫星位点的PCR扩增,在两组内共计检出8个差异性片段。FGG内,差异性片段的出现率为66.67%~86.67%;SGG内,差异性片段的出现率为40.00%~80.00%。同一位点,比较FGG和SGG组内具有差异性片段的个体,在体重、全长、体高三个性状上表现出显著差异。总计,有Poli19TUF、Poli24MHFS、Poli150TUF、Poli190TUF等4个位点与牙鲆生长性状显著相关,能够作为牙鲆良种选育研究过程中的辅助标记。

The double haploids of Japanese flounder produced by Beidaihe Central Experiment Station of Chinese Academy of Fishery Sciences were divided into fast growth group（FGG） and slow growth group（SGG） according to phenotype value of growth traits,which materials were used to carry out linkage analysis between microsatellite markers and growth traits.Twenty-two out of 24 linkage groups were covered by 40 polymorphic microsatellite loci selected from the linkage map.PCR amplification on 30 individuals from FGG and SGG were finished at 40 microsatellite loci,a total of 8 unique alleles were found in two groups.The occurrence rates of differential bands were from 66.67% to 86.67% and 40.00% to 80.00% in FGG and SGG,respectively.The individuals with differential bands in FGG and SGG at the same locus were compared.The results showed there was significant difference on body weight,total length and body depth.In total,4 loci correlated with growth traits significantly,which could be used as assisted marker during the course of selected and breeding for Japanese flounder.