摘要
建立了高效液相色谱-蒸发光散射检测法测定马鞭草药材中齐墩果酸和熊果酸含量的方法,色谱柱为PinnacleⅡC18(250mm×4.6mm,5m),流动相为甲醇-0.2%冰醋酸(体积比82.5︰17.5);淋洗速度1mL/min;柱温35℃;蒸发光散射检测器检测,漂移管温度为85℃;气体流速为2.2L/min。齐墩果酸在0.317～1.585μg范围内(r=0.9997),熊果酸在0.585～2.925g范围内(r=0.9999)线性关系良好。药材中齐墩果酸和熊果酸的回收率(n=6)分别为100.4%和97.6%,标准偏差分别为1.84%和2.34%。所建方法准确,重现性好,可作为马鞭草药材的质量控制方法。
A method for the determination of oleanolic acid and ursolic acid in Verbena officinalis L by high performance liquid chromatography-evaperative light scattering detector （HPLC-ELSD） was established. A Pinnacle Ⅱ C18 column （4.6mm×250mm） was used for separation at 35℃. The mobile phase was methanol-0.2% acetic acid （82.5 ： 17.5） with the flow rate of lmL/min. The drift tube temperature was 85℃ and the gas flow rate was 2. 2L/min. The oleanolic acid had a good linearity in the range of 0. 317 1. 585μg （ r =0. 997） and ursolic acid in the range of 0. 585-2. 925μg （ r ：0. 9999）. The average recoveries of oleanolic acid and ursolic acid were 100.4% （RSD=1.84%） and 97.6% （RSD=2.34%）, respectively. The method is accurate and repeatable and could be used for the quality control of Verbena officinalis L.
出处
《分析仪器》
CAS
2011年第5期26-29,共4页
Analytical Instrumentation
